Impact of Intramarrow Penetration on Vertical Bone Augmentation in Rabbit Calvaria

骨髓內穿通對兔子頭骨垂直骨增生效果的影響

董醒任(Shing-Zeng Dung) ； 杜裕康(Yu-Kang Tu)

《臺灣牙周病醫學會雜誌》 17卷2期 (2012/06) Pp. 109-123

摘要

The goal of the present study was to explore the impact of intra-marrow penetration on vertical bone augmentation in vivo. Five adult male New Zealand white rabbits, weighing 3 to 4 kg were used. Following anesthesia, mid-sagittal incision through the skin of the dorsum of the calvarium was performed. Treatments were divided into two groups: I) skin-periosteal flap with intra-marrow penetration (test) and II) skin-periosteal flap without intra-marrow penetration (control). A circular slit of 1.5 to 2.0 mm depth was prepared on both sides of the calvarium using a trephine drill with a diameter of 8 mm under generous irrigation with saline. The titanium domes (0.5 mm in thickness) were filled with peripheral blood for both treatment groups. After the blood clot has formed, the domes approximate 3.5 mm in inner height, were then placed in each slit by press fit. After three months, the skull bone was retrieved en bloc with the domes in situ for histological analysis. The highest point of the augmented tissue was measured directly with Boley gauge. Bone healing was evaluated by a semi-quantitative bone score according to the relative proportion of newly formed bone in the dome. Differences among means for all experiments were analyzed for statistical significance using Student's t test for matched-pair parametric data. Bone-like tissue formed on cranium in both treatment groups. Mean height of augmented tissue for Group I with decortication and Group II without decortication was 2.3 mm and 1.7 mm, respectively. The degree of vertically augmented bone height was significantly greater in the group with intra-marrow penetration than that of the control group (p=0.038). Most augmented tissue from both Groups contained 25 - 50% new bone. No statistically significant differences in bone score were noted between Groups I and II (p=0.178). In conclusion, under the limit of the preliminary experiment conditions, intramarrow penetration accelerated initial generation of bone height. Future human controlled clinical trials are encouraged to address the ability of decortication to enhance vertical bone augmentation.

關鍵字

骨髓內穿通 ； 皮質骨穿孔 ； 垂直骨增生術

並列摘要

The goal of the present study was to explore the impact of intra-marrow penetration on vertical bone augmentation in vivo. Five adult male New Zealand white rabbits, weighing 3 to 4 kg were used. Following anesthesia, mid-sagittal incision through the skin of the dorsum of the calvarium was performed. Treatments were divided into two groups: I) skin-periosteal flap with intra-marrow penetration (test) and II) skin-periosteal flap without intra-marrow penetration (control). A circular slit of 1.5 to 2.0 mm depth was prepared on both sides of the calvarium using a trephine drill with a diameter of 8 mm under generous irrigation with saline. The titanium domes (0.5 mm in thickness) were filled with peripheral blood for both treatment groups. After the blood clot has formed, the domes approximate 3.5 mm in inner height, were then placed in each slit by press fit. After three months, the skull bone was retrieved en bloc with the domes in situ for histological analysis. The highest point of the augmented tissue was measured directly with Boley gauge. Bone healing was evaluated by a semi-quantitative bone score according to the relative proportion of newly formed bone in the dome. Differences among means for all experiments were analyzed for statistical significance using Student's t test for matched-pair parametric data. Bone-like tissue formed on cranium in both treatment groups. Mean height of augmented tissue for Group I with decortication and Group II without decortication was 2.3 mm and 1.7 mm, respectively. The degree of vertically augmented bone height was significantly greater in the group with intra-marrow penetration than that of the control group (p=0.038). Most augmented tissue from both Groups contained 25 - 50% new bone. No statistically significant differences in bone score were noted between Groups I and II (p=0.178). In conclusion, under the limit of the preliminary experiment conditions, intramarrow penetration accelerated initial generation of bone height. Future human controlled clinical trials are encouraged to address the ability of decortication to enhance vertical bone augmentation.

並列關鍵字

intramarrow penetration ； decortications ； vertical bone augmentation