Purpose: The main purpose of this study is to explore (1) the palliative care and end-of-life (EoL) nursing education in Taiwan, (2) the essential EoL contents for undergraduate nursing students. Methods: We used cross-sectional, descriptive research design in this study. The 'End-of-Life Nursing Education Survey' (EoLNES) Questionnaires was used to collect data. Study subjects were nursing teachers who had delivered EoL related courses in nursing schools in Taiwan. SPSS for Windows version 19.0 was used for analyzing data. Results: Forty eight (78.7%) of 61 teachers who had delivered EoL related courses from 36 nursing schools jointed this study. Twenty nine (80.6%) nursing schools delivered 65 courses that contained EoL care issue. In average, every EoL course covered 41.7% of EoL contents. Lecture was the most common teaching method (90.8%). Almost all of the EoL content items (98.0%) listed in this study was rated as 'important' to 'very important'. Six (54.5%) of the top ten important items were in the 'physical' unit. Most of the contents were suggested to teach the fourth grade (75.5%) students, followed by third grade (24.5%). Conclusion: The findings of this study offer specific suggestions on the delivery of EoL courses in the future.