- 期刊
大豆胰蛋白酶抑制劑之抗氧化能力分析
Antioxidative Activity of Kunitz-type Trypsin Inhibitor Isolated from Soybean (Glycine max)
摘要
自由基常會引起老化作用、心血管疾病、癌症、阿茲海莫症等退化性疾病。大豆(soybean, Glycine max)，是世界普遍食用的一種食物，特別是在亞洲國家。最近研究發現，多食用大豆食品，可降低罹患骨質疏鬆(osteoporosis)、一些慢性疾病、心血管疾病及癌症。本實驗由大豆種子中分離出Kunitz-type胰蛋白酶抑制劑(Kunitz-type soybean trypsin inhibitor; SBTI)，發現其具有抗氧化能力，在試管(in vitro)試驗中，探討其對活性氧（reactive oxygenspecies, O2(上標 -•), (上標 -•)OH及H2O2）及DPPH自由基的清除能力，結果清除O2(上標 -•)自由基的IC50爲27.49±2.75μg/ml，清除OH自由基的IC50爲1.48±0.15mg/ml清除，H2O2的IC50爲2.47±0.25mg/ml，但對DPPH自由基的清除能力較弱。由結果得知，大豆Kunitz-type SBTI對活性氧具有清除的能力，特別對於清除O2(上標 -•)自由基的能力最強。
並列摘要
Reactive oxygen species and free-radical-mediated reactions have been reported in degenerative or pathological processes such as aging, cancer, coronary heart disease, and Alzheimer's disease. Meanwhile, there are many epidemiological data revealing an association between people who have a diet rich in soybean foods and a decrease in the risk of cardiovascular diseases and certain forms of cancer. In the present study, a Kunitz-type soybean trypsin inhibitor (SBTI) was isolated from soybean seed was evaluated for its in vitro scavenging effects on reactive oxygen species (O2(superscript -•), OH, and H2O2) and DPPH radical. In scavenging assays the Kunitz-type SBTI showed to be effective against all the assayed reactive oxygen species, specially for O2(superscript -•) (IC50=27.49±2.75μg/ml), OH (IC50=1.48±0.15mg/ml), H2O2 (IC50=2.47±0.25mg/ml), but displayed weak activity in the DPPH assay. These results provide scientific support for the empirical use Kunitz-type SBTI, one of the soybean seed storage proteins, may play a role as an antioxidant and may be beneficial to health when it is consumed.
被引用紀錄
延伸閱讀
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