南嶺蕘花癒傷組織誘導和不定芽形成之探討

Studies on Callus Induction and Shoot Formation in Wikstroemia indica (L.) C. A. Mey.

李佳樺(Chia-Hwa Lee) ； 張平順(Ping-Shun Chang) ； 鄭隨和(Shui-Ho Cheng)

《嘉大農林學報》 5卷1期 (2008/02) Pp. 44-56

摘要

本研究為探討南嶺蕘花(Wikstroemia indica (L.) C. A. Mey)癒傷組織誘導與不定芽形成，進行不同部位培植體及鹽類、蔗糖、生長調節劑濃度對誘導癒傷組織及不定芽影響試驗。在不同部位培植體中，節及節間培植體之癒傷組織誘導率較高，達90%，莖頂培植體之不定芽形成率最高，為90%，每個培植體平均形成3.3個不定芽。在不同鹽類及蔗糖濃度與培養條件處理，1/8 MS及添加7%蔗糖在D+D培養條件下之葉片培植體癒傷組織誘導率最高，均分別達60%及80%。另不同TDZ濃度試驗結果顯示以2.0 mg/L處理之葉片培植體癒傷組織誘導率及不定芽形成率最高，分別為80%及70%，培養45天後已有甚多癒傷組織，培養75天後已有不定芽形成。不同濃度生長調節劑處理組合影響葉片癒傷組織及不定芽的誘導，1.0 mg/L NAA+0.5 mg/L BA 及1.0 mg/L NAA+1.0 mg/L BA兩種處理組合之癒傷組織誘導率最高，均達50%，而不定芽形成率則以1.0 mg/L NAA+1.0 mg/L BA最高，為90%。0 mg/L 2,4-D+2.0 mg/L TDZ、1.0 mg/L 2,4-D+0.5 mg/L TDZ 及2.0 mg/L 2,4-D+2.0 mg/L TDZ等3種組合之癒傷組織誘導率最高，均為100%，而不定芽形成率則以不添加2,4-D而僅添加1.0 mg/L 及2.0 mg/L TDZ最高，為40%。試驗結果顯示以南嶺蕘花葉片培植體可誘導產生癒傷組織且能形成不定芽。

關鍵字

南嶺蕘花 ； 組織培養 ； 癒傷組織 ； 不定芽

並列摘要

The objective of the present study is to develop culture methods for callus induction and shoot formation in Wikstroemia indica (L.) C. A. Mey. Several factors, including different explants, different concentrations of salts, sugar, plant growth regulators, and culture conditions, possibly associated with callus and shoot induction were evaluated. Among different explants, percent callus induction from internode and node was highest, 90%. Percent shoot formation from shoot tip was top, up to 90%, the average shoots formed per explant were 3.3. In the treatments of different concentrations of salts, sugar and culture conditions, both salt strength of 1/8 MS and 7% sucrose in D+D culture condition showed highest percentage of callus induction from leaf explants, up to 60% and 80%, respectively. Treatment of 2.0 mg/L TDZ was found best in callus induction from leaf explants and shoot formation, induction percentage reached 80% and 70%, respectively. Calli were formed well after culture for 45 days and shoots were formed after culture for 75 days. Combinations of different plant growth regulators in differt concentrations do affect callus and shoot induction from leaf explants. Combinations of 1.0 mg/L NAA+0.5 mg/L BA and 1.0 mg/L NAA+1.0 mg/L BA were found best in the percentage of callus induction from leaf explants, percent induction was 50% for both. Combination of 1.0 mg/L NAA+1.0 mg/L BA showed 90% shoot formation rate which was highest. On the other hand, combinations of 0 mg/L 2,4-D+2.0 mg/L TDZ, 1.0 mg/L 2,4-D+0.5 mg/L TDZ, and 2.0 mg/L 2,4-D+2.0 mg/L TDZ had best percent callus induction from leaf explants, up to 100%. The highest percent shoot formation which is 40% was found in the treatment of 1.0 mg/L and 2.0 mg/L TDZ without 2,4-D. These results indicate that calli can be induced from leaf explants of Wikstroemia indica (L.) C. A. Mey. and shoots can be formed as well.

並列關鍵字

Wikstroemia indica L. C. A. Mey ； Tissue culture ； Callus ； Shoot