毛木耳與桂花萃取物對哺乳類細胞毒性之安全性評估

Safety Assessment of Mammalian Cytotoxicity by Extracts of Auriculariapolytricha and Osmanthusfragrans

朱紀實(Chishih Chu) ； 彭俊鈺(Jyun-Yu Peng) ； 謝佳雯(Chia-Wen Hsieh) ； 陳立耿(Lih-Geeng Chen) ； 翁博群(Brian Bor-Chun Weng) ； 洪進雄(Chin-Hsiung Hung) ； 陳俊憲(Ching-Hsein Chen)

《嘉大農林學報》 16卷1期 (2019/06) Pp. 29-44

摘要

毛木耳盛產於本校附近的嘉義縣中埔鄉，為了開發毛木耳高加價值的產品，本研究針對毛木耳烘乾與凍乾的子實體與蒂頭，以六種萃取方法，分別為冷萃、冷萃酒精沉澱、熱萃、熱萃酒精沉澱、高壓萃、高壓萃酒精沉澱，萃取出成分，再處理哺乳類中國倉鼠卵巢CHO細胞，以MTT分析細胞存活率，以評估這些毛木耳萃取方法的安全性濃度，作為後續開發毛木耳產品的依據。另外，為了開發毛木耳桂花複方產品，本研究亦對桂花進行7種方法萃取：（1）以50% EtOH reflux、（2）以EtOH reflux、（3）H_2O reflux、（4）水萃渣EtOH reflux、（5）正丁醇層、（6）EA層、（7）水層。以MTT分析對哺乳類中國倉鼠卵巢CHO細胞存活率，以評估這些桂花萃取方法的安全性濃度。結果顯示，毛木耳子實體凍乾的熱萃、高壓萃與高壓萃酒精沉澱萃取物，誘導細胞存率明顯高於控制組，這些萃取物顯示會促進哺乳類正常細胞的增殖，推測子實體的凍乾方式會比烘乾方式獲得刺激細胞增殖的成分，且熱萃、高壓萃與高壓萃酒精沉澱容易保存刺激細胞分裂增殖的毛木耳成分。桂花水層、H_2O reflux與50%EtOH reflux在1250 μg/ml的濃度以下，較不會造成細胞的毒性，未來可作為開發桂花保健成分的候選萃取方法。

關鍵字

毛木耳 ； 桂花 ； 萃取 ； 細胞存活率

並列摘要

Auriculariapolytricha(A.polytricha) is abundant in Zhongpu Township, Chiayi County near National Chiayi University.In order to develop high value products for the A. polytricha, weused six extract methods including cold extract, cold extract combined alcohol precipitation, hot extract, hot extract combined alcohol precipitation, high pressure extract, high pressure extract combined alcohol precipitation to extract the dried and freeze-dried fruiting bodies and pedicles of A.polytricha. To develop the A.polytricha combined Osmanthusfragrans(O. fragrans) compound products we used seven extracted methods including (1) 50% EtOH reflux, (2) EtOH reflux, (3) H_2O reflux, (4) water extract dregsEtOH reflux, (5) N-butanol layer, (6) EA layer and (7) water layer to extract the O. fragrans. All extractions of A.polytricha and O. fragrans were used to evaluate the cell viability of mammalian Chinese hamster ovary (CHO) cells by MTT assay. It will let us obtaina basis for the subsequent development of the A.polytricha products. Our results show that the cell viability was increased by hot extract,high pressure extract and high pressure extract combined alcohol precipitation in freeze-dried fruiting bodies of A.polytricha. These extracts could induce cell proliferation. We speculate that freeze-dried fruiting bodies will obtain more stimulators to induce cell proliferation compared with dried fruiting bodies. In addition, hot extract, high pressure extract and high pressure extract combined alcohol precipitationeasily preservethe components of the A.polytricha that stimulate cell division and proliferation. Our results also show that the cell cytotoxicity did not induce by water layer, H_2O reflux, 50% EtOHreflux of O. fragransTheseextracted-methods can be used as a candidatefor the development of health ingredients of O. fragrans in the future.

並列關鍵字

Auriculariapolytricha ； Osmanthusfragrans ； extraction ； cell viability