This study aimed to understand the firefly species composition, and appearance time and relative abundance of each species in Shekou Experimental Forest. From April 2015 to March 2016, in each month we conducted three night-time and one daytime surveys along two 1-km transects. The results showed that a total of 15 firefly species belonging to 8 genera were recorded. Among them, Abscondita cerata and Abscondita chinensis were the two most abundant species, accounting for 74.11% and 17.67% of total adult individuals recorded, respectively. The highest average number of adult firefly in each survey occurred in April and second highest occurred in June. Due to the great difference in relative abundance of each species, the overall firefly diversity in Shekou Experimental Forest is relatively low.