Six freshly frozen cadaveric shoulders were used to investigate the effect of rotator interval tear on glenohumeral stability. Our results revealed that rotator interval capsule played an important role in inferior and anterior stabilities, which were critically related to the arm rotation. Neutral and internal rotation enhanced the downward displacement when the shoulder was in hanging position. On the other hand, external rotation enhanced the anterior displacement when the shoulder was in 90˚ of abduction. In conclusion, the capsule in the rotator interval plays an important role in glenohumeral stability. Imbrication of the rotator interval capsule may help to control inferior and anterior instabilities.