- 期刊
易感基因CYP2E1、GSTM1、GSTT1基因多形性在台灣原住民、泰國及菲律賓等種族間分佈差異之研究
Ethnic Variation in CYP2E1, GSTM1, GSTT1 Genes Polymorphism Analysis: Taiwan Aborigines, Thai and Filipino
摘要
Background. Individuals metabolize chemical substrates or drugs with different the susceptibility; the differences are especially noticeable among individuals who originate from different ethic groups. Several studies have shown that the different susceptibilities could be determined using genetic polymorphisms of the enzymes which metabolized the substrate(s) or chemical(s). The polymorphisms of individuals to some cancers, in our study, we focused on the differences of these three genes in Taiwan aborigines, Thai and Fillipino populations. Method. The study population included members of various tribes of aboriginal people including the Pancah, Ayayal, Bunun, Paiwan, Puyumar, Min-nan, and natives of Thailand, and the Philippines. DNA was isolated from peripheral leukaocytes. The target DNA fragments were amplified using polymerase chain reaction, and genotype were determined using restriction fragment length polymorphism. Results. The frequency of c2 allele in members of the Pancah was 19.4%, of the Ayayal was 43.4%, of the Bununis was 29.6%, of the Paiwan was 36%, of the Puyumar was 22%, of the Min-nan was 19.6%, of the Thai was 16%, and of the Filipinos was 13.6%. The frequency of GSTM1null genotype was 56% in members of the Min-nan, 63% of the Pancah, 53% of the Ayayal, 53% of the Bunun, 42.5% of the Paiwan, 46% of the Puyumar, 48% of the Thai, and 53% of the Filipinos. The GSTT1 null genotype frequency was 44% in the members of the Min-nan, 35% of the Pancah, 52%of the Ayayal, 25% of the Bunun, 40% of the Paiwan, 41% of the Puyumar, 23% of the Thai, and 41% of the Filipinos. Conclusions. The CYP2E1-Rsal polymorphisms vary among different ethnic groups. The frequency of GSTT1 null genotype in members of the Puyumar and Thai populations were significantly lower than that of the Min-nan. The GSTM1 polymorphism was not significantly different among the populations in this study.
並列摘要
Background. Individuals metabolize chemical substrates or drugs with different the susceptibility; the differences are especially noticeable among individuals who originate from different ethic groups. Several studies have shown that the different susceptibilities could be determined using genetic polymorphisms of the enzymes which metabolized the substrate(s) or chemical(s). The polymorphisms of individuals to some cancers, in our study, we focused on the differences of these three genes in Taiwan aborigines, Thai and Fillipino populations. Method. The study population included members of various tribes of aboriginal people including the Pancah, Ayayal, Bunun, Paiwan, Puyumar, Min-nan, and natives of Thailand, and the Philippines. DNA was isolated from peripheral leukaocytes. The target DNA fragments were amplified using polymerase chain reaction, and genotype were determined using restriction fragment length polymorphism. Results. The frequency of c2 allele in members of the Pancah was 19.4%, of the Ayayal was 43.4%, of the Bununis was 29.6%, of the Paiwan was 36%, of the Puyumar was 22%, of the Min-nan was 19.6%, of the Thai was 16%, and of the Filipinos was 13.6%. The frequency of GSTM1null genotype was 56% in members of the Min-nan, 63% of the Pancah, 53% of the Ayayal, 53% of the Bunun, 42.5% of the Paiwan, 46% of the Puyumar, 48% of the Thai, and 53% of the Filipinos. The GSTT1 null genotype frequency was 44% in the members of the Min-nan, 35% of the Pancah, 52%of the Ayayal, 25% of the Bunun, 40% of the Paiwan, 41% of the Puyumar, 23% of the Thai, and 41% of the Filipinos. Conclusions. The CYP2E1-Rsal polymorphisms vary among different ethnic groups. The frequency of GSTT1 null genotype in members of the Puyumar and Thai populations were significantly lower than that of the Min-nan. The GSTM1 polymorphism was not significantly different among the populations in this study.
延伸閱讀
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