Determination of Fourteen Elements in Bone Samples Using Inductively Coupled Plasma (ICP) Analysis

以感應耦合電槳儀測定台灣人體骨中十四種元素之濃度

郭憲文(Hsein-Wen Kuo) ； 郭世明(Shyh-Ming Kuo) ； 吳錦景(Chin-Ching Wu) ； 邱明珠(Ming-Chu Chiu)

《Mid-Taiwan Journal of Medicine》 6卷3期 (2001/09) Pp. 125-132

https://doi.org/10.6558/MTJM.2001.6(3).1

摘要

目的 有關人體元素濃度之相關研究較多如血液及尿液，但人體骨中元素濃度之研究則較少，且骨中元素濃度與生理作用有關。因此，本研究之目的在評估以感應耦合電槳儀(ICP-AES)分析國人骨中之14 種元素之適當分析條件。
方法 77件骨頭樣本來自中部某醫學中心經微波消化之前處理，再以感應耦合電槳原子發射儀分析，測試三個適當儀器分析條件包括樣本吸收率，氬氣之流速及硝酸之濃度。
結果 顯示元素偵測極限範圍從1μg/L(鈷、錳及鎳)至55μg/L(鉛)，而樣本吸收率及氬氣最適流速分別為1.33 mL/min 及0.35 I/min，硝酸最適之濃度則為1M 。在人體骨中元素的量以錳(0.7μg/g)及鎘(1.2μg/g)濃度最低, 鈣(82007.9μg/g)與鎂(3005.2 μg/g)濃度最高，不過本研究骨中所含元素濃度變異性較大， 除銅原元素外，人體骨中所含元素濃度在男女性並無統計性之差異。
結論 應繼續探討影響人體骨中元素濃度之相關因素，並進一步確定以感應耦合電槳儀分析骨中元素之最適條件。

關鍵字

骨頭,感應耦合電槳(ICP)分析,多種元素

並列摘要

Objective. There have been many studies of the concentrations of elements in blood and urine, but few studies have focused on the concentrations of elements in bone. The elements in bone play, important roles in a variety of physiological processes. The objective of this study was to assess the optimum conditions for measuring the concentrations of 14 elements in human bone samples.
Methods. Seventy-seven bone samples were collected from bone surgery patients and pretreated using a microwave digest. Inductively couple plasma-atomic emission spectroscopy (ICP-AES) analysis was used to determine the optimal conditions for three variables: sample uptake rate, flow rate of Argon gas (Ar) into the nebulizer and concentrations of nitric acid.
Results. The detection limit of elements ranged from 1μg/L (Co, Mn and Mg) to 55μg/L (Pb). The optimal sample uptake rate and the optimal flow rate of Ar into the nebulizer were found to be 1.33 m1/min and 0.351/min, respectively The optimum nitric acid concentration was 1M. Mn (0.7 μg/g) and Cd (1.2 μg/g) in bone samples were present in the lowest concentrations, and Ca (82007.9 μg/g) and Mg (3005.2 μg/g) had the highest concentrations. Except for Cu, there were no significant differences in element concentrations between males and females. There was a high variation in element concentrations among the bone samples.
Conclusions. Further research is needed to investigate the factors that influence the concentrations of elements in bone. The optimal conditions for bone-element analysis using ICPAES as recommended by the authors was confirmed in this study.

並列關鍵字

bone,inductively couple plasma analysis (ICP),multi-elements