Expression of Costimulatory Molecules CD80 and CD86 in T Cells from Patients with Systemic Lupus Erythematosus

系統性紅班性狼瘡患者共同刺激因子CD80和CD86在T細胞之表現

黃春明(Chung-Ming Huang) ； 林彩秀(Tsai-Hsiu Lin) ； 王秀娟(Hsiu-Jain Wang) ； 陳季蘭(Chi-Lan Chen)

《Mid-Taiwan Journal of Medicine》 7卷1期 (2002/03) Pp. 7-13

https://doi.org/10.6558/MTJM.2002.7(1).2

摘要

背景 本篇研究在探討系統性紅斑性狼瘡患者及正常人其共同刺激分子CD80和CD86 在T細胞之表現。
方法 使用流式細胞儀(Flow cytometry)和單株抗體(monoclonal antibodies) , 去測量狼瘡患者及正常人週邊血T細胞未經及經由PHA刺激後CD80和CD86之表現。
結果 系統性紅斑性狼瘡患者其週邊血CD4+和CD8+T細胞之CD 80和CD86表現較正常人來得高。然而,只有CD4+CD80+、CD4+CD86+和CD8+CD86+三組有統計學上意 義。經由PHA刺激後, CD4+CD80+、CD4+CD86+和CD8+CD86+三組在系統性紅斑性 狼瘡患者其上升百分比在統計學上均較正常人來得低。T細胞之CD80和CD86表現和 系統性紅斑性狼瘡疾病活動度指標,如活動性指數(SLEDAI)、C3、C4及雙股核糖核 酸(dsDNA) ,皆無相關性。
結論 本篇研究顯示系統性紅斑性狼瘡患者體內之週邊單核球可表現出CD 4+ CD80+、 CD4+CD86和CD8+CD86+ .但和系統性紅斑性狼瘡疾病活動度並不相關。而在PHA 刺激後,狼瘡患者之CD4+CD80+、CD4+CD86+和CD8+CD86+明顯較正常人為低,這可能因狼瘡病人其週邊單核球有內生性缺陷,導致其活化過程改變。(中台灣醫誌2002;7:7-13)

關鍵字

活化T細胞 ； CD80 ； CD86 ； 系統性紅班性狼瘡

並列摘要

Objective. The aim of this study was to assess the effects of costimulatory molecules CD80 and CD86 expression in T cell subsets of peripheral blood mononuclear cells (PBMCs) obtained from both patients with systemic lupus erythematosus (SLE) and healthy Subjects.
Methods. CD80 and CD86 expressions in PBMCs were quantified by flow cytometry and monoclonal antibodies.
Results. Expressions of CD SO and CDs6 were higher in both CD4+ and CDS+ T cell subsets from SLE patients when compared with those from healthy subjects, although the differences were significant only in the CD4+ CD80+, CD4+ CD86+, and CD8+ CD86+ groups. After stimulation with PH A, the percentages of CD80 and CD86 in T-cell subsets were significantly lower in SLE patients compared with the contra! subjects for CD4+ CDSO+, CD4+ CDS6+ and CDS+ CDs6+. There were no significant correlations between T cell subset expressions of CO80 and CO86 and disease activity parameters which included Systemic Lupus Erythematosus Disease Activity Index (SLED AI), C3, C4 and anti-dsDNA. Conclusions. The results suggest that CD4+ CD80CD4+ CDs6+, and CD8+ CDS6+ are expressed in PBMCs of patients with SLE in vi va, but are not associated with the disease activity of SLE. PBMCs in patients with SLE may have an intrinsic defect that alters their activator process which might explain some of the T-cell immunoregulatory abnormalities observed in these patients. (Mid Taiwan J Med 2002;7:7-13)

並列關鍵字

activated T cell ； CD80 ； CD86 ； systemic lupus erythematosus