Xiao-Chai-Hu-Tang Attenuated the Expression of Proliferating Cell Nuclear Antigen and Epidermal Growth Factor in a Rat Model of Hepatic Fibrosis

小柴胡湯在大白鼠膽道結紮模型中抑制增生細胞核抗原及表皮生長因子的表現

陳明和(Ming-Ho Chen) ； 陳榮洲(Jung-Chou Chen) ； 蔡金川(Chin-Chuan Tsai) ； 王穩創(Wen-Chuang Wang) ； 謝小燕(Hsiao-Yen Hsieh)

《Mid-Taiwan Journal of Medicine》 13卷2期 (2008/06) Pp. 65-74

https://doi.org/10.6558/MTJM.2008.13(2).1

摘要

Background/Purpose. To study the effect of Xiao-Chai-Hu-Tang on the expression of proliferating cell nuclear antigen (PCNA) and to determine the relationship between the expression levels of PCNA and epidermal growth factor in a rat model of bile duct ligationinduced biliary fibrosis.
Methods. In this study, we used immunohistochemical staining to detect the expression of proliferating cell nuclear antigen in hepatocytes and bile duct epithelium in bile duct-ligated rats with or without Xiao-Chai-Hu-Tang treatment for six weeks. Reverse transcription polymerase chain reaction was used to detect the level of epidermal growth factor mRNA expression.
Results. Treatment with Xiao-Chai-Hu-Tang for six weeks reduced the expression levels of proliferating cell nuclear antigen and epidermal growth factor in hepatocytes and in bile duct epithelium. Expression of epidermal growth factor significantly correlated with the expression of proliferating cell nuclear antigen.
Conclusion. Bile duct ligation significantly induced the proliferation of hepatocytes and bile duct cells. Proliferation correlated with the increased expression of epidermal growth factor mRNA. Our data suggest that Xiao-Chai-Hu-Tang inhibits mRNA expression of epidermal growth factor, thereby reducing further hepatocellular proliferation in rats with biliary fibrosis.

關鍵字

膽道結紮 ； 表皮細胞生長因子 ； 肝纖維化 ； 增生細胞核抗原 ； 小柴胡湯

並列摘要

Background/Purpose. To study the effect of Xiao-Chai-Hu-Tang on the expression of proliferating cell nuclear antigen (PCNA) and to determine the relationship between the expression levels of PCNA and epidermal growth factor in a rat model of bile duct ligationinduced biliary fibrosis.
Methods. In this study, we used immunohistochemical staining to detect the expression of proliferating cell nuclear antigen in hepatocytes and bile duct epithelium in bile duct-ligated rats with or without Xiao-Chai-Hu-Tang treatment for six weeks. Reverse transcription polymerase chain reaction was used to detect the level of epidermal growth factor mRNA expression.
Results. Treatment with Xiao-Chai-Hu-Tang for six weeks reduced the expression levels of proliferating cell nuclear antigen and epidermal growth factor in hepatocytes and in bile duct epithelium. Expression of epidermal growth factor significantly correlated with the expression of proliferating cell nuclear antigen.
Conclusion. Bile duct ligation significantly induced the proliferation of hepatocytes and bile duct cells. Proliferation correlated with the increased expression of epidermal growth factor mRNA. Our data suggest that Xiao-Chai-Hu-Tang inhibits mRNA expression of epidermal growth factor, thereby reducing further hepatocellular proliferation in rats with biliary fibrosis.

並列關鍵字

bile duct ligation ； epidermal growth factor ； liver fibrosis ； proliferating cell nuclear antigen ； Xiao-Chai-Hu-Tang