Study on the Antioxidant Activities of Crude Extracts from the Roots of Arnebia euchroma and Lithospermum erythrorhizon

新疆紫草之抗氧化活性研究

張曼釗(Man-Jau Chang) ； 黃冠中(Guan-Jhong Huang) ； 何玉鈴(Yu-Ling Ho) ； 林宜信(I-Hsin Lin) ； 黃世勳(Shyh-Shyun Huang) ； 張恬寧(Tien-Ning Chang) ； 張恒源(Heng-Yuan Chang) ； 張永勳(Yuan-Shiun Chang)

《Mid-Taiwan Journal of Medicine》 13卷3期 (2008/09) Pp. 113-121

https://doi.org/10.6558/MTJM.2008.13(3).1

摘要

背景／目的　本研究擬探討新疆紫草及紫草之抗氧化活性及其總多酚類成分之含量，以期了解兩者間之藥理活性差異。
方法　本研究探討新疆紫草(Arnebia eucbroma (Rolye) Johnst. : AE)及紫草(Litbospermum erytbrorbizon Sieb. & Zucc.: LE)根部水及甲醇萃取物之抗氧化活性：分析方法有Trolox總抗氧化能力、DPPH染色法、清除DPPH自由基能力、還原力等，並將這些樣品進行總多酚類、類黃酮類及黃酮醇類成分含量測定，以了解其抗氧化作用與這些成分含量之間的關係。
結果　在Trolox總抗氧化能力、DPPH染色法、清除DPPH自由基能力、還原力等實驗評估上，新疆紫草(AE)之水及甲醇萃取物的活性皆大於紫草(LE)。成分測定方面，新疆紫草(AE)的兩種萃取物所含總多酚類、類黃酮類和黃酮醇類皆大於紫草(LE)。
結論　本研究證明新疆紫草之抗氧化活性大於紫草，且其活性表現與兩者所含總多酚類、類黃酮類和黃酮醇類多寡可能有密切相關。

關鍵字

抗氧化活性 ； 新疆紫草 ； 紫草 ； 總多酚類

並列摘要

Background/Purpose. To investigate the antioxidant activities and total polyphenol content in two Zicao species, Arnebia euchroma (AE) and Lithospermum erythrorhizon (LE).
Methods. The antioxidant activities of aqueous and methanolic extracts of the roots of AE and LE were evaluated by Trolox equivalent antioxidant capacity (TEAC) assay, DPPH staining, free radical scavenging capacity and reducing power methods. We also analyzed the total content of phenolic compounds, flavonols, and flavonoids to investigate whether there is a correlation between content and antioxidant activity.
Results. The Trolox equivalent antioxidant capacity and reducing power assays revealed that the methanol and water extracts of AE exhibited better antioxidant activity than those of LE. Methanol extracts of both species exhibited better antioxidant activity than water extracts. The order of antioxidant activity of the samples was AEM＞AEW≥LEM＞LEW. The DPPH radical scavenging test showed that AE exhibited greater capacity than LE to scavenge DPPH radicals. The order of scavenging ability was AEW＞AEM＞LEM＞LEW. The content of total polyphenols, flavonoids and flavonols was higher in the methanol extract than in the water extract of both species, although the content was higher in AE than in LE.
Conclusion. This study provides evidence that the antioxidant activities of AE are greater than those of LE. Furthermore, the antioxidant activities of AE and LE are closely related to the total content of polyphenols, flavonoids and flavonols.

並列關鍵字

antioxidant activites ； Arnebia euchroma ； Lithospermum erythrorhizon ； total polyphenols