以矽奈米線場效電晶體偵測癌症相關變異基因

Label-Free Biosensing of a Gene Mutation Using a Silicon Nanowire Field-Effect Transistor

吳其昌 ； 蘇丁香 ； 柯富祥 ； 劉福鯤 ； 歐耿良 ； 呂明霈 ； 潘同明 ； 莊正鏗 ； 馮思中

《國家奈米元件實驗室奈米通訊》 17卷2期 (2010/06) Pp. 25-29

https://doi.org/10.29913/NDLC.201006.0005

摘要

我們製作出一種奈米線場效電晶體(NWFET)作為生物分子感測器，利用其即時偵測、免標示及高靈敏度的特性，偵測與癌症相關的BRAF(上標 V599E)變異基因片段。當BRAF(上標 V599E)變異基因與固定在奈米線上的捕捉DNA進行雜交反應時，感測器的特性曲線會隨著DNA的雜交而產生偏移的現象；而在去除雜交的反應下，特性曲線則會回復到原來的位置。由於此奈米線感測器極為靈敏，突變基因的偵測極限可以達到10^(-15)莫耳等級。另外為了避免假陽性的現象，我們也研究了感測器專一性的辨別能力。透過免標示的奈米線感測技術，我們期許未來可以發展出偵測致癌基因的多功能生物感測器。

關鍵字

奈米線場效電晶體 ； 即時偵測 ； 免標識 ； 突變基因 ； 生物感測器

並列摘要

We have developed a silicon nanowire field-effect transistor (NWFET) device as the biosensor. The designed NWFET sensor demonstrated real-time, label-free, and high-selective properties in detecting mutation deoxyribonucleic acid (DNA). To demonstrate the sensing capacity of the NWFET, we employed the BRAF(superscript V599E) mutation gene, which correlates to the occurrence of cancers, as the target DNA sequence. The threshold voltage of the NWFET increased when the mutation gene was hybridized with the capture DNA strands on the nanowire, and decreased to the original level after de-hybridization of the gene. The sensor exhibits excellent detection property with the detection limit in sub-femtomolar level. The detection results of mismatched DNA sequences, including one-and five-base-mismatched DNA strands, could be distinguished from complementary DNA gene by this sensor. The detection results of mismatched DNA sequences could be distinguished from complementary DNA gene by this sensor. Using this label-free NWFET sensor, this method might be a potentially useful tool for biological research and oncogene screening.

並列關鍵字

NWFET ； Real-Time Detection ； Label-Free ； Mutation Gene ； Biosensor