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Effect of Extremely Low Frequency Magnetic Fields in Safety Standards on Structure of Acidophilic and Basophilic Cells in Anterior Pituitary Gland of Rats: An Experimental Study
並列摘要
After discovery of magnetite in the brain of several mammalian species including humans, it was suggested that the human brain is sensitive to magnetic fields. The present study investigated the effect of 100 μT and 500 μT extremely low frequency magnetic fields (ELF-MF), established guidelines by the International Commission on Non-Ionizing Radiation Protection (ICNIRP) for public and occupational magnetic field exposure limits at 50 Hz, on acidophilic and basophilic cells in the anterior pituitary gland of rats. 30 adult male Sprague Dawley rats were separated into three groups in this study (sham group: 10 and two study groups: 20). Rats in the study (experimental) groups separated equally into two groups that is 100 μT and 500 μT exposure groups. First experimental group rats exposed to 100 μT while second experimental group exposed to 500 μT ELF-MF for 2 hours per day (7 days in a week) during 10 months. For the sham group, the same experimental procedure was applied to the rats (2 hours / day / 7 days in a week for 10 months) except ELF-MF exposure. After ELF-MF and sham exposure, the pituitary gland were removed totally to examine the structure of acidophlic and basophilic cells in the anterior pituitary gland of rats as histomorphometric and histochemical. Affinity against dye in acidophilic and basophilic cells of first experimental groups' rat was found lower than sham group. It means that degranulation may occur in sitoplasmic granuls of these cells. Disappearing in the affinity of Periodic Acid-Shchiff (PAS) - Orange-G in acidophilic and basophilic cells of second exposure group has been indicating high disappearing of granuls in these cells. No significant differences were found between sham and exposure groups in terms of the number of acidophilic and basophilic cells in the anterior pituitary gland (p＞0.05). Although degranulation observed in acidophilic cells, the results were not found to be significant between experimental groups (p＞0.05). However, degranulated basophilic cells were found significant between experimental groups (p＜0.05). In conclusion, it was suggested that long-term ELF-MF exposure, which are the limits for public environmental and occupational magnetic field exposure guidelines, can affect affinity of PAS-Orange G in acidophilic and basophilic cells of the anterior pituitary gland in rats.
延伸閱讀
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