- 期刊
- OpenAccess
Expression of Matrix Metalloproteinase MMP-2 and Its Tissue Inhibitor TIMP-2 in Intraoral Pleomorphic Adenoma and Adenoid Cystic Carcinoma
並列摘要
Matrix metalloproteinases (MMPs) are proteolytic enzymes that are capable of degrading different substrates within extracellular matrix (ECM), and are believed to be crucial for tumor invasion and metastasis. Tissue inhibitors of MMP (TIMPs) can inhibit the action of MMPs The aim of this study was to analyze protein expression of MMP-2 and TIMP-2 in intraoral pleomorphic adenoma (PLA) and adenoid cystic carcinoma (ACC). A total of 35 formalin-fixed paraffin-embedded specimens comprising 19 PLA and 16 ACC were utilized in this study. A standard immunohistochemical technique was used to determine the expression levels of MMP-2 and TIMP-2 proteins. Sections were assessed semi quantitatively .Staining was scored as 0 (＜1% positive tumor staining), 1+ (＜25% positive tumor cells), 2+ (20-50% positive tumor cells) and 3+ (＞50% positive tumor cells). For statistical analysis, tumors were divided into two groups, low expressors (0-1+) and high expressors (2-3+). PLA showed higher TIMP-2 expression than ACC (p＜0.05). No significant difference was observed between PLA and ACC regarding MMP-2 expression. MMP-2 and TIMP-2 expressed mainly in the cytoplasm of epithelial/myoepithelial components of PLA and neoplastic epithelial cells of ACC. Myoepithelial cells may be the primary source of gelatinases in PLA and the down regulation of TIMP-2 expression in ACC might be responsible for metastasis and recurrence. The ratio value of MMP-2/TIMP-2 is valuable parameter to demonstrate the ECM degradation/deposition imbalance.
延伸閱讀
- Sun, J. (2010). Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases Are Essential for the Inflammatory Response in Cancer Cells. Journal of Signal Transduction, 2010(), 55-61. https://doi.org/10.1155/2010/985132
- 邱見怡（2009）。Study of plasma matrix metalloproteinase -2 and -9 level during concurrent chemoradiotherapy and clinicopathologic significance in patients with nasopharyngeal carcinoma〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/CSMU.2009.00025
- 曾國榮（2007）。Regulation of Extracellular Matrix Metalloproteinase-3（MMP-3）Promoter in MG-63 cells under Cyclic Tensionand Compression Force Stimulation〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2007.10566
- OguiT, R., MozetiI, V., Tešar, E. C., HupiT, D. F., MustaT, E., & Đorđević, G. (2014). Matrix Metalloproteinases 2 and 9 Immunoexpression in Prostate Carcinoma at the Positive Margin of Radical Prostatectomy Specimens. Pathology Research International, (2014), 25-32. https://doi.org/10.1155/2014/262195
- Murray, N. P., Reyes, E., Tapia, P., Badínez, L., & Orellana, N. (2012). Differential Expression of Matrix Metalloproteinase-2 Expression in Disseminated Tumor Cells and Micrometastasis in Bone Marrow of Patients with Nonmetastatic and Metastatic Prostate Cancer: Theoretical Considerations and Clinical Implications-An Immunocytochemical Study. Bone Marrow Research, 2012(), 72-80. https://doi.org/10.1155/2012/259351