IL-10 C627A Polymorphism and BMD in Postmenopausal Indonesian Women

Antonius Winoto Suhartono ； Gustivanny Dwipa Asri ； Niniarty Djamal ； Lindawati Kusdhany ； Elza Ibrahim Auerkari

《Journal of International Dental and Medical Research》 10卷S期 (2017/12) Pp. 719-722

摘要

Osteoporosis is a disease of bone metabolism marked by severe loss of bone mass, degradation of bone microarchitecture, and increased risk of fracture. The disease is typically accompanied by a 50-85% reduction in bone mineral density (BMD), which is a good predictor of the osteoporotic condition and the likelihood of fracture. The multifactorial etiology of osteoporosis is known to include genetic factors, and one of the suspected genes encodes interleukin-10 (IL-10), a cytokine that is involved in the control of both cellular and hormonal immune responses. The aim of this study was to assess the potential association of the C627A polymorphism of the IL-10 promoter with osteoporosis. For genotyping, blood serum samples were obtained from 100 consenting postmenopausal Indonesian women. The subjects included 29 control cases with a normal range of BMD, 21 cases of osteopenia, and 50 cases of osteoporosis, as classified by T-scores based on ultrasound measurement of the calcaneus bone. The genotype status of IL-10 C627A polymorphism was examined by the PCR-RFLP technique. The observed genotype fractions (CC/CA/AA, in %) were 10.3/34.5/55.2 for normal control cases, 14.3/42.9/42.9 for osteopenia cases, and 16.0/48.0/36.0 for osteoporosis cases. Generally, the genotype distributions were consistent with the Hardy-Weinberg equilibrium. The results suggest that the genotypes or alleles of the IL-10 C627A polymorphism are not significantly associated with BMD or osteoporosis risk in postmenopausal Indonesian women.

關鍵字

IL-10 ； osteoporosis ； BMD ； polymorphism ； postmenopausal