The study was carried out with coarse-grained and fine sandy oil-polluted soils in column tests and the soil was treated with the bioremediation agent SR-100. The concentrations of the hydrocarbons and anionic surfactants were determined in the fractions of soil from the column. In the experiments with the coarse-grained soil the highest concentration of residual surfactants (up to 122 mg/kg) was found in the column with unpolluted soil. The distribution of the residual surfactants was even for the fine sandy soil samples and it was slightly higher for the mixture of polluted soil and CaCO3. The results indicated degradation of surfactants in the polluted soil but the degradation did not completely remove the surfactants and leaching from soil was observed.