The purpose of this study was to explore the effect of infusion life education curriculum on in-service preschool teachers' psychological well-being. A quasi-experimental method was implemented in this study. Thirty participants in in-service preschool teachers program who took the research method course were assigned into the experiment group, while the other 30 participants from the in-service preschool teachers program were randomly assigned into the control group. The experiment group was provided with infusion life education curriculum, whereas the control group did not receive any instruction about life education. The findings indicated that the experiment group demonstrated significant psychological well-being, infusion life education curriculum had medium to large effects on in-service preschool teachers' psychological well-being. Those findings would give helpful information to preschool teacher human resource management and future study.