口腔黏膜下纖維化及口腔白斑症患者的周邊血液單核細胞TNF-α、TGF-β及IFN-r mRNA 表現量研究

mRNA Expression of TNF-α, TGF-β and IFN- r Cytokines in Peripheral Blood Mononuclear Cells in Patients with Oral Submucous Fibrosis and Leukoplakia

張松文(Sung-Wen Chang) ； 張基隆(Kee-Lung Chang) ； 陳鴻榮(Hong-Rong Chen) ； 陳中和(Chung-Ho Chen) ； 楊奕馨(Yi-Hsin Yang) ； 黃逸岳(I-Yueh Huang) ； 高郁勛(Yu-Hsun Kao) ； 謝天渝(Tien-Yu Shieh)

《Taiwan Journal of Oral Medicine & Health Sciences》 19卷1期 (2003/04) Pp. 37-47

https://doi.org/10.7059/TJOMHS.200304.0037

摘要

Oral cancer has become a serious health issue in Taiwan, and it is believed that oral submucous fibrosis (OSF) and leukoplakia are precancerous lesions. Therefore to focus on the etiology of these oral mucosal lesions is very important for the prevention and treatment of cancer. Oral precancerous mucosal lesions have a close link to the immune systems, and cytokines have played an important role on how to regulate the immune systems. It is important that human peripheral blood mononuclear cells could produce cytokines, and it has become a guideline of how to evaluate the cellular immunity of these patients. In our study, we cultured the peripheral mononuclear cells of the patients with OSF(12 persons) and leukoplakia(l2 persons) by PHA irritation for 72 hours, and then detected the mRNA expression of TNF-α、TGF- (3 and IFN-γof the mononuclear cells by RT-PCR method. Our result showed that the TNF-αmRNA (0.46+0.13 ＞ 0.36+0.13) and IFN-γmRNA (0.53+0.19＞ 0.47+0.19) expression was higher in OSF patients than the control group and TGF-β may have different expressions (0.57+0.08 ＞ 0.54+0.08) because of different stages of OSF. TNF-α mRNA (0.51 +0.13 ＞ 0.36+0.13) and IFN-γ mRNA (0.49+0.19＞ O. 47+0.19) expression was higher in leukoplakia patients but TGF- /3 mRNA expression (0.43十0.08 ＜ O.54+0.08) was lower in patients with leukoplakia than the control group.

關鍵字

口腔黏膜下纖維化 ； 口腔白斑 ； 腫瘤壞死因子 ； 轉型因子 ； 干擾素

並列摘要

Oral cancer has become a serious health issue in Taiwan, and it is believed that oral submucous fibrosis (OSF) and leukoplakia are precancerous lesions. Therefore to focus on the etiology of these oral mucosal lesions is very important for the prevention and treatment of cancer. Oral precancerous mucosal lesions have a close link to the immune systems, and cytokines have played an important role on how to regulate the immune systems. It is important that human peripheral blood mononuclear cells could produce cytokines, and it has become a guideline of how to evaluate the cellular immunity of these patients. In our study, we cultured the peripheral mononuclear cells of the patients with OSF(12 persons) and leukoplakia(l2 persons) by PHA irritation for 72 hours, and then detected the mRNA expression of TNF-α、TGF- (3 and IFN-γof the mononuclear cells by RT-PCR method. Our result showed that the TNF-αmRNA (0.46+0.13 ＞ 0.36+0.13) and IFN-γmRNA (0.53+0.19＞ 0.47+0.19) expression was higher in OSF patients than the control group and TGF-β may have different expressions (0.57+0.08 ＞ 0.54+0.08) because of different stages of OSF. TNF-α mRNA (0.51 +0.13 ＞ 0.36+0.13) and IFN-γ mRNA (0.49+0.19＞ O. 47+0.19) expression was higher in leukoplakia patients but TGF- /3 mRNA expression (0.43十0.08 ＜ O.54+0.08) was lower in patients with leukoplakia than the control group.

並列關鍵字

oral submucous fibrosis ； leukoplakia ； tumor necrosis factor ； transforming factor ； interferon