抗汞綠膿桿菌對不同重金屬生物吸附之研究及其開發微量金屬分析之應用

Study of Metal Biosorption by Pseudomona Aeruginosa PU21(Rip64) and Its Application in Novel Analysis

林漢鎮(Hann-Jenn Lin) ； 林曉青(Shau-Ching Lin)

《宜蘭技術學報》 9期工學專輯 (2002/12) Pp. 91-96

https://doi.org/10.6179/nit.bce.2002.09.18

摘要

本研究是以自製Nafion/Dithizone化學修飾電極，電化學分析法探討抗汞菌綠膿桿菌Pseudomonas aeruginosa PU21（Rip64）（死菌）對鉛、銅、鎘等離子的生物吸附、脫附與再生及探討吸附平衡動力模式。實驗參數包括接觸時間，pH值及實驗溫度的影響，尋求吸附、脫附最佳條件。在此條件下利用菌体具有吸附重金屬特性，以此菌体為重金屬離子的萃取物質，藉由吸附作用後再用少量酸沖洗脫附，使重金屬從待測物主要基質之溶液中分離出來，並且達成濃縮作用，再結合電化學微分脈衝陽極剝除伏安法測定環境水樣及生物試樣微量分析。測定結果具有高度的實用性、可靠性和精確性。

關鍵字

生物吸附 ； 微分脈衝陽極剝除伏安法

並列摘要

As described in many previous papers, biomass had ability to adsorb heavy metallic ions from aqueous solution. The adsorption due to the interaction could exist in nonviable or viable microbial cells that had been used to recover metallic ions from aqueous solution. In this study, pseudomonas aeruginosa PU21 is used to investigate the model of biosorption, desorption and regeneration of the low concentration of metal ion, such as Pb^(+2), Cu^(+2) , Cd^(+2). Experiment is conducted with various contact time, pH values, temperature and co-ions effect to obtain the optimal conditions of adsorption and desorption. Pseudomonas aeruginosa (PU21 is also be used with the electrochemicaI analysis (Differ pulse anodic stripping voltametry) method to determine the trace metals in environmental and biological samples. Reliability and accuracy of the proposed methods are confirmed by checking with standard reference materials.

並列關鍵字

Biosorption ； Differ pulse anodic stripping voltametry