Thirty percentage of chrysanthemum cultivars were mutants. In addition, the differentiation and dedifferentiation of chrysanthemum in tissue culture were regulated easily. In this paper, callus culture, derived from leaf or petal, and capitulum culture combined with irradiation of γ-ray for mutation were introduced. Furthermore, chimera petal through callus culture could regenerate plantlet with uniform petals. The mutants via mutation changed the characteristics, such as flower color, size and inflorescence. These mutation methods can induce mutants in 2 years.