培養基組成分對國蘭根莖生長與芽體分化之影響

Effect of Medium Composition on Rhizome Growth and Differentiation of Chinese Cymbidium

王才義(Tsai-Tih Wang) ； 黃瓊慧(Chiung-Hui Huang) ； 張倍瑜(Bei-Yu Chang)

《植物種苗》 11卷3期 (2009/09) Pp. 51-65

https://doi.org/10.30077/SN.200909.0004

摘要

達摩報歲蘭和彩虹四季蘭根莖培植體培養於蔗糖20和30 g/L的修改過的1/2MS（1962）培養基中，較有助於根莖的增殖與生長，每個培植體平均可產生2支根莖，且根莖長度亦較其他濃度處理者長。未添加活性碳的培養基可些微促進達摩報歲蘭和彩虹四季蘭根莖的生長，其第二分支的形成率為80%和鮮重為1110 mg，而根莖培養於含活性碳之培養基，有較高的芽體形成率，但是多數根莖褐化。將達摩報歲蘭根莖頂端培植體培養於BA 5 mg/L+NAA 0.5 mg/L的培養基中，有助於根莖增殖和芽體形成，每個月進行繼代培養一次，培植體可形成較多數量的根莖（rhizome）和根（root）。彩虹四季蘭根莖則培養於NAA 0.1 mg/L+BA5 mg/L培養基，其芽體形成率達100%，每個根莖培植體有3.3個芽體，但NAA 濃度過高時，根莖生長異常且褐化率高。

關鍵字

報歲蘭 ； 四季蘭 ； 根莖 ； 培養基 ； 器官發生

並列摘要

Cymbidium sinense 'Da-mo' and Cymbidium ensifolium 'Tsai Hung' rhizomes cultured on modified 1/2MS（1962）medium with 20 and 30 g/L sucrose, promoted rhizome proliferate and growth, produced 2 rhizomes per explant, and rhizome length were better than other concentration trentment. Rhizomes culture on media without activated charcoal could slight promoted rhizomes growth, formation percentage of 2nd branches were 80% and fresh weight were 1110 mg. Rhizomes cultured on media with activated charcoal, had high shoots formation percentage, but most rhizomes were browned. Culture medium supplemented with BA 5 mg/L+NAA 0.5 mg/L were suitable for Cymbidium sinense 'Da-mo' rhizome tip proliferate and differentiation. When rhizomes were subcultured every month, more rhizomes and roots formed. Rhizomes of Cymbidium ensifolium 'Tsai Hung' cultured on NAA 0.1 mg/L + BA 5 mg/L medium, shoot formation percentage were 100%, produced 3.3 shoots per rhizome, but high NAA concentration were produced abnormal rhizome.

並列關鍵字

Cymbidium sinense (Jackson in Andr.) Willd. ； Cymbidium ensifolium (L) Sw. ； rhizome ； culture medium ； organogenesis