藥用植物大薊組織培養大量繁殖與其染色體數之研究

Mass Propagation of Medicinal Plant, Cirsium Japonicum DC. inVitro and Its Chromosome Number

陳景明(Ching-Ming Chen) ； 葉茂生(Mau-Shing Yeh)

《植物種苗》 12卷2期 (2010/06) Pp. 33-47

https://doi.org/10.30077/SN.201006.0003

摘要

本試驗利用台灣產藥用植物大薊之莖節及葉片為培植體，探討大量繁殖之條件並觀察其染色體。結果如下：種子在1/2 MS培養基中發芽之無菌苗，取單一莖節及葉片為培植體，分別接種在MS培養基，添加0.2、0.5、1.0、2.0或4.0 mg/L BA 和0、0.1或0.5 mg/L NAA之配方並以不添加BA和NAA之培養基為對照組，共計16種培養基，分別以照光和黑暗處理，培養1個月後調查芽體再生及癒合組織形成結果，莖節和葉片培養於含有0.2 mg/L BA和0.1 mg/L NAA之培養基，照光處理者可獲得最大量增殖的芽體（莖節8.5個、葉片7.4個），而黑暗處理者皆不利芽體再生，而有利於癒合組織之形成。將初代培養所得之叢生芽體培養具2-3葉片時，將其切開成單株，接種在添加0、0.1、0.5、1.0 或2.0 mg/L NAA 之MS 與1/2 MS培養基誘導發根，1個月後在含有0.1 mg/L NAA之MS培養基最佳，平均根數為11.4條、平均根長4.3 cm、平均莖長7.3cm及平均發根率100 %。發根後之瓶苗可直接移植於泥碳土：珍珠石=1：1之栽培介質，1個月後存活率為100 %，可得到生長良好之植株。觀察經組織培養所獲得之再生和野生植株根端之染色體數，兩者均為2n=34。

關鍵字

大薊 ； 組織培養 ； 大量繁殖 ； 染色體數

並列摘要

The purpose of this study was to establish the best condition for mass propagation of medicinal plant, Cirsium japonicum DC. by tissue culture technology, and to inspect the chromosome numbers of in vitro regenerated and wild plant. The results was summarized as follows：Stem and leaf explants taken from seeds germinated in 1/2 MS medium, and cultured in basic MS medium with 16 different composed with 0.2,0.5, 1.0, 2.0, 4.0 mg/L BA and 0, 0.1, 0.5 mg/L NAA, and control without BA and NAA. The highest number of shoots (8.5 and 7.4) were obtained under MS＋0.2 mg/L BA＋0.1 mg/L NAA in light treatment after one month cultivation. The individual shoot obtained from regenerating 2-3 leafed shoots well cultured in MS and 1/2 MS medium with 0.1, 0.5, 1.0 and 2.0 mg/L NAA, respectively for root formation. After one month cultivation, the best rooting (100 %) was obtained with the MS medium containing 0.1 mg/L NAA for two explants. The average of number roots was 11.4, the average of length roots was 4.3 cm, and the average of length shoots was 7.3 cm. The rooted shoots were transplanted directly on medium of perlite and peat-moss (1:1), after one month of culturing, 100 % plants showed good growth. Chromosome number from root tip cell was found wild and in vitro plant all with the number of 2n=34.

並列關鍵字

Cirsium japonicum DC. ； In vitro ； Mass propagation ； Chromosome number