- 期刊
基因轉殖木瓜種苗快速檢測方法之研究
Study of Rapid Detection of Transgenic Papaya Seedling
摘要
基因轉殖木瓜種苗檢測時最常採用之方式是聚合酶鏈鎖反應（PCR）檢測法，針對樣品的核酸檢測目標片段，再利用電泳與膠片照相系統進行分析，其敏感度非常高，但需耗費較多的檢測時間與成本。本文研究取代一般PCR後進行電泳分析與膠片照相程序，利用Multiplex PCR、毛細管電泳、快速螢光分子檢測及Enzyme-Linked Immunosorbent Assay等方法與一般PCR檢測法比較，並評估相關成本。各方法的檢測成本均低於一般PCR。另利用Multiplex PCR技術可減少檢測次數，可節省基因轉殖木瓜檢測約50％的直接成本；開發酵素連結免疫分析法（Enzyme-Linked Immunosorbent Assay）方式可在6小時內完成檢測，並可以肉眼區分轉殖與非轉殖木瓜樣品，且無需特殊儀器。
並列摘要
The most common way of Genetically Modified (GM) or transgenic papaya inspecting method is Polymerase Chain Reaction (PCR) inspecting method, to amplify the sample target DNA fragment, then analysis with the eyectrophoresis and gel image system. The sensitivity is high, but cost more time and money. To replace the electrophoresis and gel image system after PCR, we compared the efficiency and cost with the multiplex PCR, the capillary electrophoresis (CE), and the fluorescent detector, and ELISA. Finally we found the Multiplex PCR could reduce the inspecting times. As GM papaya an example we may save about 50%. Development of immune colorimetric (ELISA) method could be detected within 6 hours to complete, and visually distinguish between GM and Non-GM papaya samples.
延伸閱讀
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