芭菲爾鞋蘭（Paphiopedilum）花粉活力檢測及貯藏之研究

Study of Pollen Viability on Paphiopedilum and Pollen Preservation

童季芬(Chi-Fen Tung) ； 劉黃碧圓(Pi-Yuan Liu Huang) ； 韓智賢(Chih-Hsien Sun) ； 蔡智賢(Jyh-Shyan Tsay)

《植物種苗》 13卷3期 (2011/09) Pp. 53-70

https://doi.org/10.30077/SN.201109.0004

摘要

利用Alexander's溶液染色法，推測芭菲爾鞋蘭Paphiopedilumniveum、P. ang-thong、P. wenshanense、P. tranlienianum、P. concolor × P. ang-thong、P. rothschildianum × P. micranthum及P. rothschildianum × P armeniacum的花粉活力。結果得知，7種供試材料之花粉活力均以盛開階段為最高，其中P. niveum、P. wenshanense、P. ang-thong及P. tranlienianum，其花粉活力均在85.1%以上，雜交種（F1）P. concolor × P. ang-thong、P. rothschildianllm × P. micranthum、P. rothschildianu × P. armeniacum，則介於42.0%─75.7% 之間。花朵老化階段的花粉活力最低，原種介於66.4%─75.1%，而雜交種（F1）則介於11.0%─52.1%之間。P. niveum、P. wenshanense、P. tranlienianum及P. ang-thong的花粉活力以下午4:00時採集者為最高，分別為97.9%、96.2%、94.7% 及90.0%。將P. tranlienianum花粉貯藏在不同溫度下處理150min，其中以25℃ 與30℃ 處理組之花粉活力較高，均在90.3% 以上，而貯藏在50℃ 的花粉活力最低，僅為60.0%。另以相同方法檢測21個原種及22個雜交種（F1）芭菲爾鞋蘭的花粉活力，其中原種P. lowii花粉活力97.1% 為最高，最低為P. primulinum其花粉活力為31.2%。22個芭菲爾鞋蘭雜交種（F1）的花粉活力,同亞屬雜交種（F1）之花粉活力介於45.1%─87.0% 之間，皆高於異亞屬（F1）雜交種的花粉活力（5.2%─40.3%）。結果顯示，芭菲爾鞋蘭異亞屬雜交其後代的花粉活力皆低於同亞屬的雜交後代。

關鍵字

亞力山大染劑 ； 雜交育種 ； 貯藏溫度 ； 花粉萌芽

並列摘要

.In this study, the Alexander's solution staining was used to test the pollen vitality of Paphiopedilum niveum, P. wenshanense, P. ang-thong, P. tranlienianum, P. concolor x P. ang-thong, P. rothschildianum x P. micranthum and P. rothschildianum x P. armeniacum, respectively. It was learned from the results that the highest pollen viability of all seven materials provided was obtained in the bloom stage, among which that of P. niveum, P. wenshanense, P. ang-thong, and P. tranlienianum were over 85.1%. Pollen viability of hybrids of (F1) as P. concolor x P. aug-thong, P. rothschildianum x P. micranthum, and P. rothschildianum x P. armeniacum were between 42.0% and 75.7%. The lowest pollen viability was shown in the aging stage of flower, between 66.4% and 75.1% for native species, and between 11 .0% and 52.1% for hybrids (F1). The pollen viability of P. niveum, P. wenshanense, P. tranlienianum, and P. ang-thon g were highest when collected at 4:00 pm, as 97.9%, 96.2%, 94.7%, and 90.0%, respectively. After storing P. tranlienianum pollens under different temperatures for 150 minutes, the pollen viability of treatments in 25°C and 30°C were higher, over 90.3%, and the lowest pollen viability was in 50°C, only 60.0%. In addition, the same method was used to test the pollen viability of 18 native species and 12 hybrids (F1) of Paphiopedilum, among which the pollen viability of native species. P. lowiii was the highest, and P. prhnulinum was the lowest as 31.2%. Among 22 Paphiopedihun hybrids (F1), the pollen viability of hybrids (F1) of same subgenres was between 45.1% and 87.0%, which was higher than that of (F1) hybrids of different subgenres (5.2%-40.3%). The results showed that the pollen viability of Paphiopedilum hybrids crossed from different subgenres were lower than that of hybrids crossed from same subgenres.

並列關鍵字

Alexander's solution ； hybride breeding ； storage temperature ； pollen germination