運動訓練對憂鬱症老鼠影響之生理機轉評估

李瑞娟 ； 劉鎮國 ； 黃順顯

《政大體育研究》 18期 (2006/03) Pp. 87-99

https://doi.org/10.30411/CTTYYC.200603_(18).0008

摘要

憂鬱已是現代社會的重要議題，目前約有2%的人有重度憂鬱的困擾，其中20%左右的人有中度憂鬱症的困擾。實驗動物中發現，早期與母親隔離的初生動物，長大後在行為上會發生深遠的影響。我們的實驗室也發現早期與母親隔離的初生動物會有憂鬱症的傾向。在過去運動曾經被拿來當作改善憂鬱傾向的方法，然而關於運動訓練如何影響大腦的訊息傳遞，目前並不清楚。本研究的目的就是要運用我們所發展出的憂鬱症老鼠之實驗模式，來探討憂鬱症的病理轉機。我們將以強迫游泳試驗來檢驗早期隔離老鼠其憂鬱症發展的情形。並檢驗運動訓練對憂鬱症老鼠在強迫游泳試驗之行為表現改善狀況。除了行為觀察外，我們同時研究腦細胞的分子機轉。我們將觀察憂鬱症老鼠其大腦訊息調控的改變。同時再比較運動訓練的老鼠，相同腦區憂鬱行為上有無差異。並探討可否直接以影像處理的方式，研究杏仁核受刺激後鈣離子訊息傳遞機轉有何差異，以做為未來憂鬱症研究的基礎。本研究發現憂鬱症的老鼠其MAPK在杏仁核的活性有變高的現象。初步的研究結果發現，在老鼠身上發現運動可以改善憂鬱的情形。同時我們也可以直接測定鈣離子在杏仁核的濃度變化，故此運動模式值得繼續應用在憂鬱症的研究上，深入研究運動訓練的機轉。

關鍵字

憂鬱 ； 運動訓練 ； MAPK激酶

並列摘要

Mood disorders are among the most prevalent forms of mental illness. Sever forms of depression affect 2-5%of the U.S. population and up to 20% of the population suffer from milder forms of the illness. Numerous studies have shown that early life stress in nonhuman primates produces profound and long-lasting changes in behavior ad biological function. Our previous data indicated that the maternal deprivation animal showed a depression-like behavior in response to force swimming tests. Studies on human depression revealed beneficial effects of exercise in the treatment of psychiatric disorders. However, the underlying mechanisms are largely unknown. In this study, the underlying mechanisms of depression will be test in normal, as well ad maternal deprivation rats with or without exercise training. The force-swimming test will be carried out to test the animal behavior before and after exercise treatments. The brain slice calcium image of amygdala will also be investigated for the possible application of future investigation of depression. Our study revealed that amygdala phospho-MAPK level was elevated in maternal deprivation rats. Exercise training can inhibited the immobility time during forced swim test, suggesting a inhibition on depression-like behavior through exercise training. Calcium image of amygdala were also accessible by brain slice preparation. Our data provided a basic foundation for on the application of exercise program on depression treatments.

並列關鍵字

Zno ； depression ； exercise training ； MAPK