【論文摘要】Effects of Repetitive Hyperbaric Oxygen Therapy on Neuroprotection in Middle Cerebral Artery Occlusion Rats

【論文摘要】重複性高壓氧治療對中大腦動脈阻塞大鼠神經保護之效果

張恆誌(Heng-Chih Chang) ； 楊雅如(Yea-Ru Yang) ； 王瑞瑤(Ray-Yau Wang)

《物理治療》 45卷2期 (2020/06) Pp. 162-162

https://doi.org/10.6215/FJPT.202006.P35

摘要

Background and Purpose: Hyperbaric oxygen (HBO) intervention has been indicated to be one of possible therapies for central nerve system injury. However, the effects of HBO after brain ischemia were inconstant and the underline mechanisms were not fully known. The purposes of present study were to investigate the effects of repetitive HBO intervention in a transient middle cerebral artery occlusion (MCAO) animal model. Methods: 72 Sprague-Dawley rats received transient MCAO and randomly assigned to the normal air control or HBO intervention groups. Each group was further divided into 3 subgroups according to the intervention time period (7, 14, and 21 days). HBO was started at 24h post-MCAO for 60 min once per day. At 24h post-MCAO and the end of the finial intervention, rats received motor behavior test to examine the motor performance. At the end of experiment, half of rats in each subgroup were sacrificed under anesthesia and the right motor cortex was removed for examining the expressions of p-Akt, glutathione (GSH), and activities of glutathione peroxidase (GPx) and reductase (GR). Another half of rats in each subgroup were used for examining the infarct volume. Results: Rats received 14 and 21 days of HBO intervention showed significantly reduced in the infarct volume, and increased in the expressions of p-Akt, GSH, and activities of GPx and GR. The motor performance also showed significantly improved in rats received HBO for 14 and 21 days. There were no significant changes in the normal air control and HBO for 7 days groups. Conclusion: Repetitive HBO intervention for at least 14 days in transient brain ischemic rats provided neuroprotective effects through modulating the cell survival pathway and antioxidative defense system. Clinical Relevance: Our results provide important information about the use of repetitive HBO starting from the acute phase post brain ischemia. These results may also provide for the clinical studies to investigate the therapeutic effects for patients.