Background and Purpose: Previous studies have shown that the tightness of pectoralis minor (Pm) and the imbalanced strength of periscapular muscles (Ps) may associate with the poor posture and scapular dyskinesis (ScD). Strengthening exercise is commonly used to enhance the strength of Ps while the self-massage maneuver was generally recommended for releasing the tightness of Pm. The eccentric exercise can lengthen the shortened muscle but seldom applied on the tightened Pm. Therefore, the aims of this study were to compare the effects of self-massage, eccentric, and Ps strengthening on Pm length and Ps strength as well as the influence on posture changes in college students with ScD. Methods: Nineteen collegiate students with ScD were recruited and allocated into three groups, including eccentric exercise for Pm (ECPm), strengthening exercise for Ps (STPs), and self-massage for Pm (SMPm) groups, to receive 4-week intervention. The subjects performed posterior tilting and external rotation of scapula eccentrically with elastic band as ECPm. For STPs, the control exercises for middle and lower trapezius and serratus anterior were executed. The SMPm group used a massage ball to release Pm. The length of Pm, forward shoulder angle and maximal strength of shoulder rotators and Ps were measured before and after interventions. The Wilcoxon signed rank test was used to compare the intervention outcomes in each group. The Kruskal-Wallis test was used to compare the changes after intervention between groups. Results: The length of Pm was increased significantly after training with ECPm (15.90 ± 0.69 cm vs. 17.12 ± 0.58 cm, p = 0.018) and STPs (15.62 ± 0.66 cm vs. 16.10 ± 0.67 cm, p = 0.028). The increment of Pm length with ECPm was larger than that with the other interventions. The strength of lower trapezius was increased with ECPm (22.10 ± 12.08 lb vs. 27.17 ± 11 lb, p = 0.028) and with STPs (19.44 ± 9.28 lb vs. 24.10 ± 10.58 lb, p = 0.046). There was no significant change of posture in each group. Conclusions: The shortness of Pm and/ or the weakness of Ps cause the SD, which should be evaluate comprehensively for an effective intervention. Clinical Relevance: The ECPm and STPs may have better effects on Pm length and strength of Ps than the SMPm alone.