Background and Purpose: Glenohumeral internal rotation deficit (GIRD) is known as a risk factor of shoulder injuries, caused by the tightness of capsule and muscle on posterior shoulder and the increased humeral retroversion (HR). However, the evaluation may put more attention on the soft tissue than on the bony changes of humerus. This study aimed to compare the HR between bilateral shoulders and to examine the relationship between HR and the deficit of internal rotation (IR) in participants with GIRD. Methods: Nine collegiate students (5 males and 4 females, mean age 23.0 ± 1.2 years) with GIRD (the difference was of internal rotation between bilateral shoulders was more than 20°) were recruited. After the horizontal alignment of humeral head was monitored by sonography, the HR was measured as the glenohumeral IR in supine with 90° of shoulder abduction and 90° of elbow flexion. The Wilcoxon sign rank tests was used to compare the HR between two shoulders. The Spearman's correlation was used to examine the relationship between IR deficit and HR. Results: The IR deficit was occurred on the dominant shoulder in all participants (range of IR, dominant side: 50.0° ± 9.7°, non-dominant side: 74.4° ± 10.1°). The HR on the dominant shoulder (66.0° ± 8.7°) was significantly larger than that on the non-dominant side (58.0° ± 8.1°). A significant correlation was found between the IR deficit and the HR (ρ = 0.65, p < 0.01). Conclusions: The degree of deficit in IR correlates with the HR in participants with GIRD. The HR should be considered before improving the range of internal rotation. Clinical Relevance: Both the soft-tissue tightness and HR should be evaluated to realize the respective contributions to IR deficit.