運動調控基因表現：與健康相關性之研究

The Exercise Regulating Gene Expression: The Relationship with Health

李麗珍(Li-Chen Lee) ； 陳慕聰(Mu-Tsung Chen) ； 黃宏裕(Hung-Yu Huang) ； 陳宗與(Chung-Yu Chen) ； 蘇福新(Fu-Hsin Su)

《大專體育學刊》 7卷2期 (2005/06) Pp. 287-299

https://doi.org/10.5297/ser.200506_7(2).0023

摘要

先前的流行病學研究顯示，運動與身體的健康狀態具有高度的關聯性，但其詳細的分子機轉仍尚未被瞭解。目前基因晶片技術已經逐漸成熟，然而是否能夠利用基因晶片作為有效的分析工具用於探討運動與基因表現間的關聯性，目前仍不清楚。50毫升的血液樣本抽取自規律運動習慣者與靜態生活型態者之靜脈血液，經純化RNA後，以含有人類8,000點基因之ABC Human Universo-Chip基因晶片進行cDNA microarray技術進行基因表現分析。結果發現，有規律運動習慣者在預防感冒、抑制癌症發生與血紅素合成相關基因表現顯著高於無規律運動習慣者；然而，規律運動與否與這些基因的關聯性仍須待後續系統性的研究進行聯結與證實。

關鍵字

運動 ； 基因表現 ； 健康 ； 基因晶片

並列摘要

Previous epidemiological studies have shown that exercise is closely associated with the whole body health condition, whereas the molecular-based evidence of this interaction still has not been adequately understood. Recently, the gene microarray technology has already matured for clinical practice; however, whether gene microarray could be a reliable analytical tools for discovering the relationship between exercise and health remains unclear. We obtained 15 mL venous whole blood from two different group of subjects with various physical activity levels (regular physical activity and sedentary). After we extracted the total RNA from the blood samples, we analyzed the disease-related gene expression by the microarray kit with 8,000 genes (ABC Human Universo-Chip). We found that the expression of those genes related to preventing influenza, inhibiting tumor growth, and hemoglobin synthesis are significantly higher in regular exercise groups than the sedentary ones. However, whether exercise really results in those benefits requires further systematic studies.

並列關鍵字

exercise ； gene expression ； health ； microarray

參考文獻

Boord, J. B.,Fazio, S.,Linton, M. F.(2002).Cytoplasmic fatty acid-binding proteins:Emerging roles in metabolism and atherosclerosis.Current Opinion in Lipidology.13(2),141-147.

Booth, F. W.,Chakravarthy, M. V.,Gordon, S. E.,Spangenburg, E. E.(2002).Waging war on physical inactivity: Using modem molecular ammunition against an ancient enemy.J. Appl. Physiol..93(1),3-30.

Boule, N. G.,Haddad E.,Kenny, G. P.,Wells, G. A.,Sigal, R. J.(2001).Effects of exercise on glycemic control and body mass in type 2 diabetes mellitus: A meta-analysis of controlled clinical trials.JAMA.286(10),1218-1227.

Bronikowski, A. M.,Carter, P. A.,Morgan, T. J.,Garland, T.,Ung Jr, N.,Pugh, T. D.,Weindruch, R.,Prolla, T. A.(2003).Lifelong voluntary exercise in the mouse prevents age-related alterations in gene expression in the heart.Physiol. Genomics.12,129-138.

Calvert, P. M.,Frucht, H(2002).The genetics ofcolorectal cancer.Ann. Intern. Med..137,603-612.