補充靈芝對衰竭運動後老鼠抗氧化能力及肌肉損傷之影響

本研究旨在探討補充靈芝對衰竭運動後老鼠體內抗氧化能力及肌肉損傷之影響。以 40 隻雄性老鼠為研究對象，隨機分成控制組、衰竭運動組、靈芝組和靈芝運動組，共四組，各 10 隻。補給靈芝的組別每天以每公斤體重 40 mg/kg 劑量餵食老鼠，為期四週；運動組於最後一週進行跑步機適應，隔天以漸增式衰竭運動跑至衰竭。採二因子變異數分析來考驗靈芝補充與衰竭運動對老鼠血液中肌酸激酶、乳酸去氫酶、丙二醛、過氧化物歧化酶、榖胱甘肽過氧化酶活性之影響。結果發現衰竭運動組的肌酸激酶、乳酸去氫酶、丙二醛活性顯著高於控制組，過氧化物歧化酶及榖胱甘肽過氧化酶活性則顯著低於控制組 (p < .05)。且靈芝運動組乳酸去氫酶、丙二醛活性皆顯著低於衰竭運動組 (p < .05)，過氧化物歧化酶活性則顯著高於衰竭運動組 (p < .05)，肌酸激酶與榖胱甘肽過氧化酶則無顯著差異 (p > .05)。由上述總結，補給靈芝能有效防止衰竭運動導致過氧化物歧化酶活性之降低及乳酸去氫酶與丙二醛活性升高之情形，有助於增強老鼠體內之抗氧化能力，減緩因衰竭運動導致氧化傷害及肌肉損傷之情形。

關鍵字

肌酸激酶；乳酸去氫酶；丙二醛；過氧化物歧化酶；榖胱甘肽過氧化酶

並列摘要

This study was designed to investigate the effects of Ganoderma lucidum supplementation on antioxidant status and muscular damage after exhaustive exercise in rat. Forty male Sprague-Dawley rats were randomly divided into four groups: control (C, n=10), exhaustive exercise (E, n=10), Ganoderma lucidum treated (G, n=10) and Ganoderma lucidum plus exhaustive exercise (GE, n=10). Ganoderma lucidum was supplemented at the dose of 40 mg/kg body weight for four weeks. Exhaustive exercise was performed on a rodent treadmill with the progressive protocol. Two way ANOVA was carried out to examine the effects of exhaustive exercise and Ganoderma lucidum supplementation on creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and malondialdehyde (MDA) levels. The activities of CK, LDH and the levels of MDA in E group were significantly (p＜.05) higher than C group, while the activities of SOD and GPx were significantly (p＜.05) lower than C group. However, LDH activity and MDA level were significantly lower in GE group compared to E group, and SOD activity was significantly increased with Ganoderma treatment compared to E group. Results from our study demonstrate that Ganoderma lucidum supplementation is beneficial to increase antioxidant status, and reduce the elevation of oxidative stress and muscular damage in exhaustive exercised rats.