短期槲皮素補充對腳踏車衰竭運動引起氧化壓力與發炎反應之影響

本研究目的在探討槲皮素補充對腳踏車衰竭運動引起氧化壓力、發炎反應的影響和運動至衰竭時間效果。招募10名健康受試者參與本次隨機單盲交叉試驗，受試者分別接受連續7天口服（1,000 mg/day）槲皮素或安慰劑膠囊補充，兩次試驗至少間隔7天。受試者於7天補充後，隔天實驗日早上（300卡輕食進食後）在原地腳踏車上進行單次75%最大攝氧量（maximal oxygen uptake, VO_(2max)）強度至衰竭表現之時間測試，單次腳踏車衰竭運動前後分別收集血液樣本，分析總抗氧化能力（total antioxidant capacity, TAC）、超氧化物歧化酶（superoxide dismutase, SOD）、丙二醛（malondialdehyde, MDA）、發炎反應細胞激素（interleukin-6, IL-6）、腫瘤壞死因子-α（tumor necrosis factor-α, TNF-α）和肌肉損傷指標肌酸激酶（creatine kinase, CK）、肌紅蛋白（myoglobin, Mb）。研究結果顯示7天槲皮素補充明顯提升受試者腳踏車運動至衰竭時間，同時明顯增加SOD活性，降低運動MDA濃度，運動後槲皮素補充明顯減緩運動引起IL-6增加幅度。同樣地，CK反映趨勢和IL-6相同。然而，發炎指標TNF-α和肌肉損傷指標Mb則沒有明顯改變。本研究推論連續7天口服槲皮素補充明顯提升運動衰竭時間，可能和提升身體抗氧化能力，減緩運動引起氧化壓力、促發炎程度和肌肉損傷有關。

關鍵字

超氧化物歧化酶；丙二醛；細胞激素IL-6 ；腫瘤壞死因子-α ；肌酸激酶

並列摘要

The purpose of this study was to investigate the effect of 7-d oral quercetin supplementation on cycling time to exhaustion, and cycling-induced oxidative stress and inflammation in adults. Ten healthy adults were recruited to perform a randomized single blind cross-over study with 7-d quercetin (1,000 mg/day) and placebo supplementation, which was separated by a 7-d washout period. All participants performed a single bout of cycling exercise challenge at 75% maximal oxygen uptake (VO_(2max)) and time to exhaustion was recorded after completion of 7-d quercetin or placebo supplementation in a randomized order. Blood samples were taken before and after exercise challenge to assess the total antioxidant capacity (TAC), superoxide oxide dismutase (SOD), and malondialdehyde (MDA) levels. Inflammatory biomarkers, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), creatine kinase (CK), and myoglobin (Mb) were also determined in the blood. The results showed that time to exhaustion was significantly higher in quercetin trial compared to those in placebo. Oral quercetin supplementation significantly increased SOD activity and decreased MDA levels after 75% VO_(2max) cycling exercise challenge. Exercise-induced IL-6 elevation was significantly attenuated, and CK was also similarly responded to quercetin supplementation. However, there were no significant differences in TNF-α and Mb levels between the trials. We conclude that short-term oral quercetin supplementation enhances cycling performance of adults, which may be due the increased antioxidant capacity and attenuated exercise-induced oxidative stress, pro-inflammatory response and muscle damage.

並列關鍵字

superoxide dismutase ； malondialdehyde ； interleukin-6 ； tumor necrosis factor-α ； creatine kinase

參考文獻

Chang, W.-H., Hu, S.-P., Huang, Y.-F., Yeh, T.-S., & Liu, J.-F. (2010). Effect of purple sweet potato leaves consumption on exercise-induced oxidative stress and IL-6 and HSP72 levels. Journal of Applied Physiology, 109(6), 1710-1715. doi:10.1152/japplphysiol.00205.2010

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Davis, J. M., Murphy, E. A., Carmichael, M. D., & Davis, B. (2009). Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 296(4), R1071- R1077. doi:10.1152/ajpregu.90925.2008

Graefe, E. U., Wittig, J., Mueller, S., Riethling, A. K., Uehleke, B., Drewelow, B., ... Veit, M. (2001). Pharmacokinetics and bioavailability of quercetin glycosides in humans. The Journal of Clinical Pharmacology, 41(5), 492-499. doi:10.1177/00912700122010366

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國際替代計量

短期槲皮素補充對腳踏車衰竭運動引起氧化壓力與發炎反應之影響

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