單重態氧對運動中的生理效益

Physiological Benefits of Singlet Oxygen During Exercise

洪敦賓(Tun-Pin Hung) ； 張健忠(Jian-Chung Chang) ； 林亦香(I-Hsiang Lin) ； 巫錦霖(Ching-Lin Wu)

《大專體育學刊》 Volume 26 Issue 4 (2024/12) Pp. 383-396

https://doi.org/10.5297/ser.202412_26(4).0005

Abstracts

氧氣在激發狀態可以形成單重態氧（singlet oxygen, ¹O₂），運動中吸入¹O₂可能有助於運動效率。本研究旨在製造一個簡單的¹O₂產生器（singlet oxygen generation, SOG），驗證其產生效果，並以人體非最大運動測試，探討對運動中的生理效益。研究方法：1.SOG裝置：利用光感物質亞甲藍，以紅光的曝光劑可激活空氣中的氧氣，進而產生大量的¹O₂，並以1,3-二苯基異苯並呋喃的含量變化來確定¹O₂的產量。2.人體運動測試：招募12名受試者，以單盲方式進行兩次非最大運動測試，隨機分派到¹O₂與一般環境，運動中分析攝氧量、心跳率、血乳酸與運動自覺量表。實驗結果顯示：1.SOG確實能有效的產生¹O₂。2.人體運動測試顯示，在¹O₂環境下，相同的非最大運動強度下有顯著較低的攝氧量、心跳率與血乳酸濃度。本研究簡單的SOG，吸入所產生的¹O₂，可增加氧氣利用效率，可能可以促進運動中的有氧能量路徑，降低血乳酸，並提升非最大運動表現。

Keywords

非最大運動 ； 攝氧量 ； 心跳率 ； 血乳酸

Parallel abstracts

Oxygen in its excited state can form singlet oxygen (¹O₂). Inhaling ¹O₂ during exercise may contribute to exercise efficiency. This study aimed to describe the development of a simple ¹O₂ generation (SOG) device, verify its effectiveness in producing ¹O₂, and assess its physiological benefits during submaximal exercise in humans. Method: (a) SOG device: Utilizing the photosensitizer methylene blue (MB) and exposure to red light, oxygen in the air can be activated to produce singlet oxygen. The production of singlet oxygen is determined by monitoring changes in the concentration of 1,3-diphenylisobenzofuran (DPBF). (b) Human exercise testing: Twelve participants were recruited for two single-blind submaximal exercise tests, randomized in both ¹O₂ and regular environments. Oxygen consumption, heart rate, blood lactate, and rating of perceived exertion were analyzed during exercise. Results showed that: (a) The SOG device effectively produced ¹O₂. (b) Results from human exercise testing showed significantly lower oxygen consumption, heart rate, and blood lactate concentration during submaximal exercise in the ¹O₂ environment. In conclusion: The simple SOG device, when inhaled, improved oxygen utilization efficiency, potentially enhancing aerobic energy pathways during exercise and reducing blood lactate levels, thereby improving submaximal exercise performance.

Parallel keywords

submaximal exercise ； oxygen consumption ； heart rate ； blood lactate

References

Chin, K. K.,Trevithick-Sutton, C. C.,McCallum, J.,Jockusch, S.,Turro, N. J.,Scaiano, J. C.,Foote, C. S.,Garcia-Garibay, M. A.(2008).Quantitative determination of singlet oxygen generated by excited state aromatic amino acids, proteins, and immunoglobulins.Journal of the American Chemical Society.130(22),6912-6913.

Costill, D. L.,Coyle, E.,Dalsky, G.,Evans, W.,Fink, W.,Hoopes, D.(1977).Effects of elevated plasma FFA and insulin on muscle glycogen usage during exercise.Journal of Applied Physiology.43(4),695-699.

Costill, D. L.,Fink, W. J.(1977).Muscle glycogen sparing during exercise in man.Medicine & Science in Sports & Exercise.9(1),47.

Coyle, E. F.(1995).Substrate utilization during exercise in active people.The American Journal of Clinical Nutrition.61(4),968S-979S.

D’Autréaux, B,Toledano, M. B.(2007).ROS as signalling molecules: Mechanisms that generate specificity in ROS homeostasis.Nature Reviews Molecular Cell Biology.8(10),813-824.