八角蓮癒傷組織之誘導與鬼臼素之檢測

Callus Induction of Podophyllum pleianthum Hance and the Detection of Podophyllotoxin

陳靜怡(Chin-Yi Chen) ； 李昆達(Kung-Ta Lee) ； 劉祖惠(Tsu-Hwie Liu) ； 劉文雄(Wen-Hsiung Liu)

《臺灣農業化學與食品科學》 42卷6期 (2004/12) Pp. 412-420

https://doi.org/10.6578/TJACFS.2004.053

摘要

八角蓮(Podophyllum pleianthum)為台灣民間治療毒蛇咬傷與跌打損傷之傳統草藥，並含有重要抗腫瘤成分－鬼臼素。由於八角蓮生長緩慢並受到大量採集，在台灣已瀕臨絕種。本研究之目的，在利用八角蓮無菌植株為材料，探討癒傷組織之誘導條件，並檢測癒傷組織是否含有鬼臼素。結果顯示，於含有2-3 mg/L 2,4-D之B5基礎培養基，可使根部培值體產生癒傷組織，其誘導率為30%；而以10-15 mg/L NAA取代2,4D，則可促進來自莖部（含莖頂）之培植體產生癒傷組織，誘導率可達90％。將誘導所得之癒傷組織經冷凍乾燥與甲醇萃取處理後進行HPLC分析檢測，發現由根部誘導生成之癒傷組織含有鬼臼素。此成分之停滯時問（10.1分鐘）及UV吸收光譜，與鬼臼素標準品一致。

關鍵字

八角蓮 ； 鬼臼素 ； 癒傷組織

並列摘要

Podophyllurn pleianthum Hance has been traditionally used in Taiwan for healing snake bitten and wounds. It is also valuable for containing podophyllotoxin-an precursor of effective anti-tumor agents treated in clinical medicine. Due to slow-growing habit and over-collection, P. pleianthum is rare from its natural habitation. We report here the results of callus induction of P. pleianthum from different parts of regenerated plantlet. Our data revealed that solid medium containing B5 salts with 2-3 mg/L 2,4-D was essential for root callus formation, but the induction rate was as low as 30%. When presence of 10-15 mg/L NAA, it was more effective than 2,4-D for initiating callus from stems of P. pleianthum, and the induction rate reached 90%. Methanol extraction of callus derived from different parts of P pleianthuin was further analyzed by HPLC (equipped with a photodiode array detector) for podophyllotoxin detection. A metabolite, extracted from root-induced callus, appeared at the same time (10.1 min) with the same UV absorption spectra as authentic podophyllotoxin was observed.

並列關鍵字

Podophyllum pleianthum ； Podophyllotoxin ； Callus induction