Cultural Conditions for Degradation of Benzoate by a Mutant of Corynebacterium pseudodiphtheriticum

探討利用Corynebacterium pseudodiphtheriticum NTU-7C降解苯甲酸之培養條件，得知於合成培養基中添加苯甲酸，對羥苯甲酸、磺基水楊酸及兒茶酚可同時誘導生產參與降解苯甲酸最初反應之苯甲酸1，2-二加氧酶及NADH-2，6-二氯酚靛酚(DCIP)還原酶活性。增加苯甲酸之添加量可提高苯甲酸酶之活性最高至5克／公升，但增加苯甲酸之添加量對NADH-DCIP還原酶之活性並無影響。添加腖可顯著促進細胞生長，當攪拌速率設定在400 rpm時，在培養8及9小時可獲得最高之苯甲酸1，2-二加氧酶及NADH-DCIP還原酶之活性，分別為37單位／公升及390單位／公升。而醱酵液中之苯甲酸在培養14小時後可完全被降解。依據上述結果顯示，醱酵液中之苯甲酸濃度及溶氧控制乃是誘導生產苯甲酸1，2-二加氧酶與NADH-DCIP還原酶及影響降解苯甲酸最初反應之最重要因子。

關鍵字

苯甲酸；苯甲酸1,2-二加氧酶； NADH-DCIP還原酶；棒狀桿菌；深部培養

並列摘要

The culture conditions for degradation of benzoate by Corynebacterium pseudodiphtheriticum NTU-7C were investigated. It was found that benzoate 1,2-dioxygenase and NADH-2,6-dichlorolindophenol (DCIP) reductase activities which were involved in the initial reaction for the degradation of benzoate were simultaneously induced by the addition of benzoate, p-hydroxybenzoate, sulfosalicylic acid or catechol in a synthetic medium. The increase of benzoate 1,2-dioxygenase activity was paralleled by an increase in the concentration of benzoate up to 5 g/L. The increase of NADH-DCIP reductase activity was maintained despite the high concentration of benzoate. The cell growth was greatly promoted by the addition of peptone. When the agitation speed was fixed at 400 rpm, the maximum activities of benzoate 1,2-dioxygenase and NADHDCIP reductase reached about 37 U/L and 390 U/L at 8 h-and 9 h-cultivation, respectively. Benzoate in the fermentation culture was completely consumed within 10 h. It was concluded that the concentration of benzoate and the control of dissolved oxygen in the fermentation culture are the most important parameters for the production of benzoate 1,2-dioxygenase and NADH-DCIP reductase, and for the initial reaction of benzoate degradation.

並列關鍵字

Benzoate ； Benzoate 1,2-dioxygenase ； NADH-DCIP reductase ； Corynebacterium ； submerged culture

國際替代計量

Cultural Conditions for Degradation of Benzoate by a Mutant of Corynebacterium pseudodiphtheriticum

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