幾丁聚醣對糖尿病大白鼠血糖及血膽固醇濃度之影響

Effect of Chitosan on Plasma Cholesterol and Glucose Concentration in Streptozotocin-induced Diabetic Rats

姚賢宗(Hsien-Tsung Yao) ； 黃杉頁(Shan-Ye Huang) ； 江孟燦(Meng-Tsan Chiang)

《臺灣農業化學與食品科學》 44卷2期 (2006/04) Pp. 122-132

https://doi.org/10.6578/TJACFS.2006.015

摘要

為了解幾丁聚醣對糖尿病大白鼠血糖及血膽固醇的影響，以雄性Sprague-Dawley(SD)大白鼠為試驗動物，分別進行兩個試驗，試驗(Ⅰ)：大白鼠經皮下注射streptozotocin(STX；60m/kg B.W.)誘發糖尿病，試驗分成2組，分別為(1)糖尿病纖維素組及(2)糖尿病幾丁聚醣組，共進行3週。結果發現，糖尿病幾丁聚醣組雖不改變體重與攝食量，但有較低的飲水量與排尿量。此外，幾丁聚醣會明顯降低血糖、血膽固醇濃度及降低肝臟膽固醇含量並增加糞便膽固醇的排泄。值得注意的是幾丁聚醣會明顯降低小腸雙醣酶(lactase and maltase)活性。試驗(Ⅱ)：將大白鼠分成四組分別為：(1)纖維素組(2)幾丁聚醣組(3)糖尿病纖維素組及(4)糖尿病幾丁聚醣組，共進行7週。結果發現，幾丁聚醣並不會改變正常大白鼠血糖濃度，但會顯著的降低糖尿病大白鼠血糖、血膽固醇及游離脂肪酸濃度。無論在正常或糖尿病大白鼠，幾丁聚醣皆會明顯降低肝臟膽固醇及三酸甘油酯含量。幾丁聚醣也會降低糖尿病大白鼠血漿中thiobarbuturic acid reactive substance(TBARS)值。另一方面，幾丁聚醣會提高糖尿病大白鼠肝臟中六碳醣激酶活性。由本研究結果推測，幾丁聚醣可能具有調節糖尿病大白鼠血膽固醇及血糖濃度的作用。

關鍵字

幾丁聚醣 ； 膽固醇 ； 血糖 ； 雙醣酶 ； 糖尿病大白鼠

並列摘要

To investigate the effect of chitosan on plasma cholesterol and glucose level in streptozotocin (STZ)-induced diabetic rats, male Sprague-Dawley rats were used as experimental animals. The present study was divided into two parts. Exp (Ⅰ): The diabetic rats were performed by subcutaneous injection of streptozotocin (60mg/kg B.W.), and then divided into two groups. They are (1) diabetic cellulose group (DCE) and (2) diabetic chitosan group (DCS). The experiment was done for three weeks. Although there was no significant difference in body weight and food intake, lower drinking and urine volume were observed in diabetic rats fed the chitosan diet when compared with those animals fed the cellulose diet. Lower levels of plasma glucose, plasma cholesterol, liver cholesterol and higher fecal cholesterol concentration excretion were found in diabetic rats fed the chitosan diet. It was noteworthy that the intestinal disaccharidase (lactase and maltase) activity was significantly decreased after the chitosan treatment. Exp (Ⅱ): Rats were divided into two groups of normal rats and two groups of diabetic rats, and fed a cellulose or chitosan diet for seven weeks. Results showed that chitosan did not alter plasma glucose concentration in normal rats but dramatically reduced plasma glucose, cholesterol and free fatty acid levels in diabetic rats. Chitosan also reduced hepatic cholesterol and triglyceride contents in both normal and diabetic rats. Lower plasma thiobabituric acid reactive substance (TBARS) value was significantly decreased in diabetic rats after the chitosan treatment. In addition, chitosan caused an increase in hepatic hexokinase activity in diabetic rats. Results from this study suggest that chitosan may play an important role in regulation of plasma cholesterol and glucose concentrations in diabetic rats induced by streptozotocin.

並列關鍵字

Chitosan ； Cholesterol ； Glucose ； Disaccharidase ； Diabetic rats