餵食松杉靈芝對BALB/c小鼠免疫調節的影響

The Immunomodulatory Effects of Ganoderma tsugae Supplementation on Immune Responses of BALB/c Mice

陳妙齡(Miaw-Ling Chen) ； 林金源(Jin-Yuarn Lin) ； 林璧鳳(Bi-Fong Lin)

《臺灣農業化學與食品科學》 44卷4期 (2006/08) Pp. 243-249

https://doi.org/10.6578/TJACFS.2006.028

摘要

本研究目的為探討靈芝子實體對動物整體免疫反應的影響，分别以管餵方式給予BALB/c小鼠補充松杉靈芝YK01舆YK07雨種産品六週俊，测定吞噬細胞及自然殺手細胞活性，分析血清抗體濃度，以及脾臟細胞的增生能力舆細胞激素之分泌量，以評估松杉靈芝對於全身性免疫反應之調節作用。結果顯示，松杉靈芝YK01可以顯著的增加週邊血液中吞噬細胞含量、活化自然殺手細胞、促進IgM舆IgG抗體生成，同峙能促進脾臟細胞分泌IL-2，而抑制IL-4分泌。松杉靈芝YK07则顯著促進血清IgG舆IgA的生成、脾臟T細胞的增生，且同樣促進IL-2分泌且抑制IL-4的分泌。此外，補充松杉靈芝均顯著的降低肝臟TBARS的含量，降低脂質過氧化的程度。由以上結果得知，不同的松杉靈芝産品，對於免疫反應的調節功效舆免疫反應指標的影響有所不同。不過，整體而言，本研究所使用的松杉靈芝産品，可顯著降低肝臟過氧化物指標，對於適應性免疫反應具有活化舆調節的作用。

關鍵字

松杉靈芝 ； 自然殺手細胞活性 ； 吞噬作用 ； 免疫調節

並列摘要

To investigate the immunomodulatory effect of fruit body of Ganoderma tsugae on immune responses in animal model, BALB/c mice were tube-fed with different Ganoderma tsugae products YK01 and YK07 daily for 6 weeks and killed for further analysis of phagocytic activity, natural killer cell activity, serum antibody, and cell proliferation and cytokine secretion of splenocytes. The results showed that the low dosage YK01 group significantly increased NK cell activity, the percentage of granulocyte, immunoglobulin (Ig) G and M. T cell proliferation and IL-2 secretion of spenocytes were significantly higher but IL-4 secretion was lower in the Ganoderma tsugae YK01 group. Furthermore, Ganoderma tsugae could decrease the lipid peroxidation level in mice liver. The YK07 group had significantly higher serum IgG and IgA, T cell proliferation and IL-2 secretion of spenocytes, but lower IL-4 secretion. In addition, both YK01 and YK07 significantly decreased hepatic TBARS values, suggesting their antioxidant effect. Therefore, different Ganoderma tsugae products may exert different effects on immune responses. However, the products used in this study both showed antioxidant effect and immunomodulatory effect on adaptive immune response.

並列關鍵字

Ganoderma tsugae ； NK cell activity ； Phagocytosis ； Immunoregulation