二十二碳六烯酸對Pentylenetetrazol(PTZ)誘發大鼠癲癇造成神經膠細胞凋亡作用及空間記憶之影響

Effect of Docosahexaenoic Acid on Apoptosis of Glial Cells and Spatial Learning in Rats with Pentylenetetrazole-induced Epileptic Seizure

蘇正任(Jheng-Ren Su) ； 陳志誠(Chih-Cheng Chen) ； 吳美莉(Mei-Li Wu) ； 陳柏榮(Bo-Zon Chen) ； 莊秀琪(Hso-Chi Chaung)

《臺灣農業化學與食品科學》 44卷4期 (2006/08) Pp. 272-281

https://doi.org/10.6578/TJACFS.2006.032

摘要

In the present study, we investigated the beneficial effects of docosahexaenoic acid (DHA) supplementation on detriments in central nervous system (CNS) by using pentylenetetrazol (PTZ)-induced seizure in neonatal rats as an animal model. To prove this hypothesis, we induced seizure attacks on ten-day-old post-natal (P10) rats and examining their glial cells' apoptosis and spatial learning before or after treated with 0.4% fish oil (FO) or corn oil (CO). In experiment 1 (Exp 1), the dietary treatment was undertaken on the same day as PTZ-induced seizure until animals were sacrificed for examining the protection of DHA in fish oil from the brain injury in PTZ-induced seizure. Meanwhile, the prevention effect of fish oil on CNS damage was evaluated by giving the dietary treatment for consecutive 7 days before PTZ-induced seizure in experiment 2 (Exp 2). The results showed significant increases of DHA percentages in phospholipids of brain tissue and decreased apoptosis of oligodendrocytes and microglia cells in FO in Exp 1 (p＜0.05). The percentages of DHA were negatively correlated with the apoptosis of oligodendrocytes in Exp 1 (r=-0.61, p=0.02). The mean percentage of total swimming time in target region as escape latency in FO tended to be longer than that in CO in both of Exp 1 and 2. These results implicate that DHA protects the glial cells from apoptosis in brain injury caused by PTZ-induced seizure and thus improves special learning activities.

關鍵字

細胞凋亡 ； 二十二碳六烯酸 ； 寡樹突神經膠細胞 ； 小神經膠細胞 ； 癲癇

並列摘要

In the present study, we investigated the beneficial effects of docosahexaenoic acid (DHA) supplementation on detriments in central nervous system (CNS) by using pentylenetetrazol (PTZ)-induced seizure in neonatal rats as an animal model. To prove this hypothesis, we induced seizure attacks on ten-day-old post-natal (P10) rats and examining their glial cells' apoptosis and spatial learning before or after treated with 0.4% fish oil (FO) or corn oil (CO). In experiment 1 (Exp 1), the dietary treatment was undertaken on the same day as PTZ-induced seizure until animals were sacrificed for examining the protection of DHA in fish oil from the brain injury in PTZ-induced seizure. Meanwhile, the prevention effect of fish oil on CNS damage was evaluated by giving the dietary treatment for consecutive 7 days before PTZ-induced seizure in experiment 2 (Exp 2). The results showed significant increases of DHA percentages in phospholipids of brain tissue and decreased apoptosis of oligodendrocytes and microglia cells in FO in Exp 1 (p＜0.05). The percentages of DHA were negatively correlated with the apoptosis of oligodendrocytes in Exp 1 (r=-0.61, p=0.02). The mean percentage of total swimming time in target region as escape latency in FO tended to be longer than that in CO in both of Exp 1 and 2. These results implicate that DHA protects the glial cells from apoptosis in brain injury caused by PTZ-induced seizure and thus improves special learning activities.

並列關鍵字

Apoptosis ； Docosahexaenoic acid ； Oligodendrocytes ； Microglia cells ； Seizure