篩選調節過敏反應食材之細胞培養及小鼠評估方法

Evaluation of the Regulatory Effects of Dietary Materials on Allergic Immune Responses by in vitro and in vivo Assay

陳妙齡(Miaw-Ling Chen) ； 林璧鳳(Bi-Fong Lin)

《臺灣農業化學與食品科學》 45卷6期 (2007/12) Pp. 316-326

https://doi.org/10.6578/TJACFS.2007.036

摘要

已知許多菇菌類食材具有免疫調節的功能，本研究以松杉靈芝、巴西洋菇、茯苓及樟芝等菇菌類爲主要篩選材料，採用肥大細胞株RBL-2H3、BALB/c鼠脾臟細胞及DO11.10小鼠(卵蛋白特異性T細胞受器基因轉殖鼠)脾臟T細胞等培養方式進行篩選，再以抗原致敏動物餵食模式評估細胞培養篩選方式，建立並評估有效的篩選方法。結果顯示，松杉靈芝及巴西洋菇不影響RBL-2H3肥大細胞分泌組織胺，但茯苓及樟芝處理促進組織胺分泌。菇菌類處理可促進BALB/c鼠脾臟細胞分泌IL-2，並抑制IL-4分泌。以卵蛋白特異性Th1或Th2細胞進行評估，菇菌類顯著抑制Th2細胞激素分泌；並抑制卵蛋白T輔助細胞分化傾向Th2反應。動物試驗結果顯示，松杉靈芝、巴西洋菇及茯苓餵食降低卵蛋白致敏小鼠肺沖洗液中IL-5與嗜伊紅白血球聚集，並抑制Th2細胞激素分泌。綜合本研究結果，菇菌類可顯著減緩致敏小鼠呼吸道發炎，且抑制IL-4及IL-5等Th2細胞激素生成。卵蛋白特異性T輔助細胞與待測樣品共同培養，並分析細胞激素分泌量作爲Th1及Th2之分化導向指標，是較爲省時且有效的篩選方式。可作爲食材調節過敏免疫反應之有效篩選方法。

關鍵字

肥大細胞 ； T輔助細胞 ； 組織胺 ； 細胞激素 ； 菇菌類

並列摘要

Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan (Accepted for publication: November 28, 2007) in this study, we like to establish both in vitro and in vivo systems to study and explore the possible functional foods to regulate the allergic immune responses. It has been well documented that fungi might exert immuneomodulatory effect, and thus the Ganoderma tsugae, Agaricus blazei, Poria cocos and Antrodia camphorate were tested for allergic immunomodulatory effect, by using RBL-2H3 mast cell line, primary splenocyte form BALB/c, and OVA-specific CD4+ T cells from T cell receptor (TCR) transgenic DO11.10 mice (naïve OVA-specific T cells). The results showed that ionomycin-stimulated histamine productionform RBL-2H3 cells did not affect by Ganoderma tsugae and Agaricus blazei, but increased by oria cocos and Antrodia camphorate. OVA-stimulated IL-2 secretion form Th1 cells were increased, but IL-4 and IL-5 secretion from Th2 cells was decreased by all the fungi samples. Furthermore, fungi treatment increased the IL-2 level and decreased Th2 cytokines production during naïve OVA-specific T cell differentiation in vitro. To further investigate the effect of fungi on airway inflammation, the allergen-sensitized mice challenged with aerosol allergen supplemented with Ganoderma tsugae, Agaricus blazei or Poria cocos. The data showed that the percentage of eosinophils, IL-5 and total protein content in bronchoalvelor lavage fluid (BALF), and IL-4, IL-5 levels produced by splenocytes were significantly decreased in mice fed with Ganoderma tsugae, Agaricus blazei and Poria cocos. Overall, these data suggested that the OVA-specific Th cell cultured with OVA plus samples, which could be as a screening method for anti-allergic immune responses substance.

並列關鍵字

RBL-2H3 cell ； OVA-specific Th cell ； Histamine ； Cytokines ； Mushroom