餵食樟芝子實體對於小鼠體內肝腫瘤細胞之免疫調節與抑制腫瘤效果之探討

Immunomodulatory and Anti-tumor Effects of Oral Administration with Antrodia cinnamomea Fruiting Bodies in BALB/c Mice

陳盈宜(Ying-Yi Chen) ； 張慧欣(Hui-Hsin Chang) ； 李曉媛(Hsiao-Yuan Lee) ； 許輔(Fuu Sheu)

《臺灣農業化學與食品科學》 46卷2期 (2008/04) Pp. 87-95

https://doi.org/10.6578/TJACFS.2008.011

摘要

The objective of this study was to investigate the immunomodulatory and anti-tumor effects of Antrodia cinnamomea fruiting bodies using a hepatoma-bearing mouse model. Oral administration of high (100 mg/kg B.W.) and medium (33 mg/kg B.W.) doses of A. cinnamomea fruiting bodies significantly increased the life spun of ATCC BNL IMEA.7R.1 hepatoma-bearing mice (3×10^2 cells/mouse) by 47.4 and 57.1%, respectively. In naive (un-injected with tumor cells) mice, orally administered A. cinnamomea fruiting bodies at high, medium, and low (11 mg/kg B. W.) doses significantly enhanced the production of TNF-α by peritoneal macrophages (p＜0.05). Oral administration of A. cinnamomea fruiting bodies at medium and low doses also significantly enhanced the production of nitric oxide by peritoneal macrophages (p＜0.05). In addition, peritoneal macrophages obtained from A. cinnamomea fruiting bodies-fed naive mice had increased cytotoxicity against hepatoma cells. In hepatoma-bearing mice, oral administration of A. cinnamomea fruiting bodies significantly increased the serum levels of tumor-specific IgG (p＜0.05). Splenocytes obtained from A. cinnanmomea fruiting bodies-fed heputoma-bearing mice had increased tumor-specific proliferation and upregulated production of IFN-γ, IL-2, and TNF-α, as well as enhanced cytotoxicity against hepatoma cells, These results indicated that A. cinnamomea fruiting bodies carried out anti-tumor activity by activating both non-specific and tumor-specific immunity.

關鍵字

樟芝 ； 子實體 ； 抑制腫瘤作用 ； 免疫調節 ； 小鼠BNL 1MEA 7R.1肝腫瘤細胞

並列摘要

The objective of this study was to investigate the immunomodulatory and anti-tumor effects of Antrodia cinnamomea fruiting bodies using a hepatoma-bearing mouse model. Oral administration of high (100 mg/kg B.W.) and medium (33 mg/kg B.W.) doses of A. cinnamomea fruiting bodies significantly increased the life spun of ATCC BNL IMEA.7R.1 hepatoma-bearing mice (3×10^2 cells/mouse) by 47.4 and 57.1%, respectively. In naive (un-injected with tumor cells) mice, orally administered A. cinnamomea fruiting bodies at high, medium, and low (11 mg/kg B. W.) doses significantly enhanced the production of TNF-α by peritoneal macrophages (p＜0.05). Oral administration of A. cinnamomea fruiting bodies at medium and low doses also significantly enhanced the production of nitric oxide by peritoneal macrophages (p＜0.05). In addition, peritoneal macrophages obtained from A. cinnamomea fruiting bodies-fed naive mice had increased cytotoxicity against hepatoma cells. In hepatoma-bearing mice, oral administration of A. cinnamomea fruiting bodies significantly increased the serum levels of tumor-specific IgG (p＜0.05). Splenocytes obtained from A. cinnanmomea fruiting bodies-fed heputoma-bearing mice had increased tumor-specific proliferation and upregulated production of IFN-γ, IL-2, and TNF-α, as well as enhanced cytotoxicity against hepatoma cells, These results indicated that A. cinnamomea fruiting bodies carried out anti-tumor activity by activating both non-specific and tumor-specific immunity.

並列關鍵字

Antrodia cinnamomea ； Fruiting bodies ； Anti-tumor effect ； Immunomodutatory effect ； Mouse BNL IMEA.7R.1 hepatoma cell line