複方中草藥抗氧化效應及對肝癌細胞之影響

The Effects of Antioxidative Activity and Human Hepatoma Cells of Chinese Herbal Teas

李嘉浩(Jia-Hao Li) ； 張瑞郎(Ruey-Lang Chang) ； 吳思敬(She-Ching Wu)

《臺灣農業化學與食品科學》 47卷3期 (2009/06) Pp. 145-154

https://doi.org/10.6578/TJACFS.2009.017

摘要

本研究以藥典記載具有保肝功能之中草藥。探討兩種複方飲品的抗氧化效應及對肝癌細胞之影響。一種複方含有靈芝、菊花、桑葉、枸杞、薄荷及白茅根（靈芝茶），另一則為黨參、苡仁、黃精、黃耆、茯苓、陳皮及枸杞（元氣飲）。抗氧化活性成分（總酚和類黃酮含量）分析中，元氣飲分別為18.38及6.43mg/g extract；靈芝茶則為27.33及10.76mg/g extract。元氣飲與靈芝茶於清除DPPH自由基能力劑量為3.0mg/mL時皆可達90%以上，而螯合亞鐵離子能力分別為9.33及48.7%。總抗氧化能力於3.0mg/mL劑量分別為斜84.8及99.7%。清除超氧陰離子之能力在5.0mg/mL劑量時，具有73.3及75.7%清除效果。兩種複方中草藥茶均能提升胞內GSH reductase與GSH peroxidase之酵素活性且能抑制肝癌細胞之存活率，其中以靈芝茶之效果較佳，於5.0mg/mL劑量下，肝癌細胞存活率下降至33.2%。兩種複方中草藥茶使肝癌細胞之細胞週期大量累積於G0/G1期，顯示能調控細胞週期之進行，並且抑制肝癌細胞之增生能力。綜合上述結果，兩種複方飲品具有抗氧化效應、提升細胞內抗氧化酵素活性及抑制肝癌細胞增生能力，顯示兩種中草藥複方飲品具有成為保健食品之潛力。

關鍵字

靈芝茶 ； 元氣飲 ； 安全性 ； 抗氧化性 ； GSH reductase ； GSH peroxidase ； 細胞週期

並列摘要

In the present study, the hepatoprotective effect of two kinds of beverages mentioned in the pharmacopoeias were investigate, which included analysis of antioxidant ability and inhibitor of cancer cell proliferation for the Hep G2 cell. One formula contained Ling Chih (Ganoderma lucidum), Ju Hua (Chrysanthemum morifolium Ramat.), Sang Ye (Morus alba), Gou Qi (Lycium chinensis), Bo He (Mentha spicata), and Bai Mao Gen (root of Imperata cylindrica); therefore, abbreviated as Ling-Chih Tea. The other formula contained Dang Shen (Codonopsis pilosula), Yi Ren (seed of Cots laciyma-jobi), Huang Jing (Polygonatum stbiricum), Huang Qi (Astragalus membranaceu), Fu Ling (Poria cocos), Ju Pi (Pericarpium of Citri Reticulata), Bo He (Mentha spicata) and Gou Qi (Lycium chinensis); therefore, abbreviated as Gen-ki Drink. The effect of antioxidative activity and inhibited cancer cell proliferation of the two compound Chinese herb teas were evaluated, respectively. In the antioxidant aspect, the DPPH radical scavenging activity of Genki Drink and Ling-Chih Tea reached 91.0 and 91.4%; whereas the ferrous ion chelating activity reached 9.33 and 48.7% respectively in the concentration of 3.0 mg/mL. The total antioxidant activity reached 84.8 and 99.7% at 3.0 mg/mL. The superoxide anion scavenging ability was 73.3 and 75.7% at 5.0 mg/mL. The total phenolics and flavonoids content of Gen-ki Drink reached 18.38 and 6.43 mg/g extract; whereas Ling-Chih Tea reached 27.33 and 10.76 mg/g extract, respectively. Furthermore, the result of the intracellular antioxidant enzyme activities showed both Chinese herb teas could raise the activity of GRd and GPx. The result of inhibition of hepatoma cell (Hep G2) viability showed both teas reduced the viability of Hep G2 cells, and Ling-Chih Tea exhibited stronger efficiency (the viability was 33.2% at 5.0 mg/mL after treated for 24 hours). The analysis of cell cycle showed a great quantity of accumulation at G0/G1 phase after treated with the two Chinese herb teas, which illustrated the inhibition of hepatoma cell proliferation was subject to the progress modification of cell cycle. In summary, the two compound Chinese herb teas showed antioxidant activity and inhibition of hepatoma cell proliferation in vitro assays. As a result, the two compound Chinese herb teas exhibited great potential as new nutraceuticals.

並列關鍵字

Ling-Chih Tea ； Gen-ki Drink ； safety ； antioxidant ability ； GSH reductase ； GSH peroxidase ； cell cycle