Excessive intake of trans fatty acids may increase the levels of blood and cholesterol LDL-C (low density lipoprotein cholesterol), leading to an increased LDL-C/HDL-C ratio and risk of cardiovascular diseases. The objectives of this study were to investigate the contents of trans fatty acids and saturated fatty acids in commercial food products by using GC method. The effects of different thermal processing methods (including roasting, microwave heating and UV irradiation) on trans fatty acid formation in beef slices were also investigated. The results showed that cream and shortening contained higher levels of trans fatty acids. The higher crisp baking foods possess a higher shortening or hydrogenated oil content. For beef slices being roasted by charcoal fire and infrared ray stove, their trans fatty acids content were no significantly increased with increasing roasting time. While beef slices were treated with microwave heating and UV irradiation, their trans fatty acid content were found to be decreased due to the oxidation of unsaturated fatty acid.