以甘油與植物油利用脂解酶之轉酯化反應生產1，3-雙醯甘油

油脂代替品廣泛應用於食品中，可減少熱量之攝取，並降低肥胖症、痛風、心血管等疾病之罹患機率。本研究利用未固定化脂解酶：脂解酶來自Mucor miehei (Mm)與Rhizopus oryzae (Ro)及固定化脂解酶：Lipozyme RM IM與Novozym 435，以三醯甘油與甘油之轉酯化作用，進行1，3-雙醯甘油之生產。以常壓、減壓及有機溶劑添加與否三種轉酯化反應模式進行探討，其中以常壓轉酯化反應模式可得較高1, 3-雙醯甘油(Diacylglycerol; DAG)生成量。主成分為單元不飽和脂肪酸之油脂（橄欖油、苦茶油及芥花油），在甘油與油脂莫耳比1:1條件下；主成分為多元不飽和脂肪酸油脂（芝麻油及葵花油），在甘油與油脂莫耳比1:2條件下，其1，3-DAG生成量介於31.2~44.7%；而甘油與油脂莫耳比2:1條件下，1，3-DAG生成量則低於20%。在60℃ 、三醯甘油與甘油莫耳比為1:1時，經Novozyme 435轉酯化可得1，3-雙醯甘油產物40%。在熱傳導試驗，以60% DAG油脂油炸薯條，有較高熱傳導速率

關鍵字

雙醯甘油；固定化脂解酶；轉酯化反應

並列摘要

Fat substitutes have been extensively used in foods to reduce calorie energy uptake, and prevent obesity, gout and cardiovascular diseases. In this study, we used the non-immobilized enzymes: lipase from Mucor miehei (Mm) and Rhizopus oryzae (Ro), and immobilized enzymes: Lipozyme RM IM and Novozyme 435, to transesterify glycerol and triglycerides to 1, 3-diacylglycerol (DAG). Three different processes for transesterification were carried out under the conditions of atmosphere with or without organic solvent, and reduced pressure without organic solvents, respectively. Higher amount of 1, 3-DAG was produced by transesterification under atmospheric pressure without addition of organic solvent. As the molar ratio of glycerol to triglycerides was 1:1 for monosaturated oils (olive, teaseed and canola oils) and 1 : 2 for polyunsaturated oils (sesame and sunflower oils), the yield of 1, 3-DAG was in the range of 31.2~44.7%. However, as the molar ratio of glycerol to triglycerides increased to 2:1, the yield of 1, 3-DAG was lower than 20%. In the case of transesterification using Novozyme 435 at 60℃ , the yield of 1, 3-DAG reached 40% under 1/1 molar ratio of glycerol/triglycerides. In the thermal conduction study, higher thermal conduction rate was detected when oils containing 60% of 1, 3-DAG was used to fry French fries.

並列關鍵字

Diacylglycerol ； Immobilized lipase ； Transesterification

國際替代計量

以甘油與植物油利用脂解酶之轉酯化反應生產1，3-雙醯甘油

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