Lyophilization and hot-air drying methods were applied to utilize broiler and silkie fowl blood, which were by-products from slaughter houses, as blood meal samples. Peptide content and ACE inhibitory activity of broiler and silkie fowl blood meal were investigated. Results showed that the silkie fowl blood meal had significantly higher peptide contents than broiler meal (p<0.05), and the lyophilization method was contributed to higher peptide contents in blood meal (p<0.05). In ACE inhibitory activity, the silkie fowl blood meal obtained from lyophilization method (SBL) showed the best inhibitory activity (38.91%) and the broiler blood meal obtained from hot-air drying method was poor (5.11%). According to the results, SBL was applied to determined the antihypertensive activity in animal model (SHR). The result showed that a significant reduction (25-30 mmHg) was observed in systolic blood pressure of SHRs after orally administrated for 4 hours. The chronic experiment also indicated oral administration of SBL (100 mg/kg bw/day) for 8 weeks improved symptom of hypertension in SHRs. Moreover, SBL did not influence blood pressure of normotension rats (WKY) in the same dose (100 mg/kg bw/day). These results suggested that lyophilized silkie fowl blood meal had high potential to be developed as the functional food with antihypertensive activity.
Lyophilization and hot-air drying methods were applied to utilize broiler and silkie fowl blood, which were by-products from slaughter houses, as blood meal samples. Peptide content and ACE inhibitory activity of broiler and silkie fowl blood meal were investigated. Results showed that the silkie fowl blood meal had significantly higher peptide contents than broiler meal (p<0.05), and the lyophilization method was contributed to higher peptide contents in blood meal (p<0.05). In ACE inhibitory activity, the silkie fowl blood meal obtained from lyophilization method (SBL) showed the best inhibitory activity (38.91%) and the broiler blood meal obtained from hot-air drying method was poor (5.11%). According to the results, SBL was applied to determined the antihypertensive activity in animal model (SHR). The result showed that a significant reduction (25-30 mmHg) was observed in systolic blood pressure of SHRs after orally administrated for 4 hours. The chronic experiment also indicated oral administration of SBL (100 mg/kg bw/day) for 8 weeks improved symptom of hypertension in SHRs. Moreover, SBL did not influence blood pressure of normotension rats (WKY) in the same dose (100 mg/kg bw/day). These results suggested that lyophilized silkie fowl blood meal had high potential to be developed as the functional food with antihypertensive activity.