- 期刊
食品中氯黴素之液相層析串聯質譜儀檢測
Determination of Chloramphenicol Residues in Foods by Liquid Chromatography-Electrospray Tandem Mass Spectrometry
摘要
氯黴素為－廣效性抗生素，可有效的抑制微生物之感染，本研究之目的係為建立以液相層析串聯質譜儀檢測食品中氯黴素之方法。分析材料以乙酸乙酯經由液相－液相萃取淨化方式取得；移動相溶液以0.1%甲酸溶液及甲醇進行梯度分析，使用層析管柱為Hypersil C18，3μm，內徑2.1×50mm，以電灑負離子模式選擇母離子m/z 321及主要子離子片段為m/z 152、257及194，以m/z 152為定量子離子，利用離子片段訊號之滯留時間、訊號面積及子離子間訊號強度比值作為定性及定量之依據。結果顯示，利用此法可得到良好之回收率與淨化效果，具有良好之再現性，平均回收率於雞肉、魚肉、蝦肉及蜂蜜中分別為90.1%、78.3%、76.2%與109.3%。
並列摘要
Chloramphenicol is a broad spectrum antibiotic, widely used as an anti-microbial agent. A liquid chromatography method with tandem mass (LC/MS/MS) by using electrospray negative ion mode detection and identification is presented for the determination of chloramphenicol in foods. The analytes were extracted with ethylacetate and defatted with n-hexane by liquid-liquid extraction. The column was a Hypersil C18 (2.1×50 mm, 3 μm) with a mixture of 0.1% formic acid water and methanol by gradient mobile phase. The electrospray negative ionization produced the parent molecular ions at m/z 321 and produced three characteristic transition reaction fragments (m/z 321 →152, 257 and 194). The recovery rates of the developed method were 90.1%, 78.3%, 76.2%, and 109.3% in chicken meat, fish meat, shrimp meat and honey, respectively.
延伸閱讀
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