酵素水解烏骨雞爪應用於抑制酪胺酸酶活性之研究

Application of the Enzymatic Hydrolysates from Silky Fowl Feet to Inhibit Tyrosinase Activity

蔡佩潔(Pei-Chieh Tsai) ； 鄭富元(Fu-Yuan Cheng) ； 柳育澤(Yu-Tse Liu) ； 劉思安(Su-Ann Liu) ； 林亮全(Liang-Chuan Lin)

《臺灣農業化學與食品科學》 49卷1期 (2011/02) Pp. 26-32

https://doi.org/10.6578/TJACFS.2011.004

摘要

The aim of this study was to investigate the functional characteristics of two-stage enzymatic hydrolysates from silky fowl feet treated with Alcalase (A), Protease N (P), and Flavorzyme (F). Three combinations, AF, PA and PF, were used. After hydrolyzing for 4 hours, the hydrolysates were lyophilized and analyzed for the soluble protein, the peptide content, UV absorption spectrum, the melanin content and the ability to inhibit tyrosinase activity. The results showed that after enzyme treatment, the soluble proteins and peptide contents of the hydrolysates were decreased and increased, respectively. This result indicates that all the enzyme combinations possess good hydrolyzing ability. All the hydrolysates had UV absorption and tyrosinase inhibitory activity (PA had the highest absorption and tyrosinase inhibitory activity). In the melanin content analysis, three hydrolysates contained about 1.92-2.32 mg/g melanin. Silky fowl feet hydrolysates have high potential to be utilized for production of the tyrosinase inhibitory peptides as a photo protection ingredient of cosmetic.

關鍵字

烏骨雞爪 ； 水解物 ； 胜肽 ； 酪胺酸酶抑制力

並列摘要

The aim of this study was to investigate the functional characteristics of two-stage enzymatic hydrolysates from silky fowl feet treated with Alcalase (A), Protease N (P), and Flavorzyme (F). Three combinations, AF, PA and PF, were used. After hydrolyzing for 4 hours, the hydrolysates were lyophilized and analyzed for the soluble protein, the peptide content, UV absorption spectrum, the melanin content and the ability to inhibit tyrosinase activity. The results showed that after enzyme treatment, the soluble proteins and peptide contents of the hydrolysates were decreased and increased, respectively. This result indicates that all the enzyme combinations possess good hydrolyzing ability. All the hydrolysates had UV absorption and tyrosinase inhibitory activity (PA had the highest absorption and tyrosinase inhibitory activity). In the melanin content analysis, three hydrolysates contained about 1.92-2.32 mg/g melanin. Silky fowl feet hydrolysates have high potential to be utilized for production of the tyrosinase inhibitory peptides as a photo protection ingredient of cosmetic.

並列關鍵字

Silky fowl feet ； Hydrolysates ； Tyrosinase inhibitory activity