以液相層析串聯質譜儀檢測雞蛋及鮮乳中殘留之離子攜帶型抗生素

Determination of Five Polyether Ionophore Antibiotics in Egg and Fresh Milk by Liquid Chromatography-Electrospray Tandem Mass Spectrometry

唐玉芸(Yu-Yun Tang) ； 盧信芳(Hsin-Fang Lu) ； 林旭陽(Hsu-Yang Lin) ； 鐘月容(Yueh-Jong Chung-Wang) ； 施養志(Yang-Chih Shih) ； 黃登福(Deng-Fwu Hwang)

《臺灣農業化學與食品科學》 50卷2期 (2012/04) Pp. 78-87

https://doi.org/10.6578/TJACFS.2012.009

摘要

本試驗方法使用液相層析串聯質譜儀（liquid chromatography/tandem mass spectrometry, LC/MS/MS），以電灑游離正離子法，進行雞蛋及鮮乳中五種離子攜帶型抗生素之檢測及方法開發。試驗步驟為取全蛋及鮮乳加入無水硫酸鈉，混合均勻直到呈現小顆粒球狀，以乙腈萃取後，經氮氣濃縮後，選用XTerraTM Phenyl之層析管柱，搭配0.1%甲酸水溶液與乙腈進行LC/MS/MS分析。此方法之雞蛋及鮮乳平均回收率介於90.4~95.0%及95.0~104.7%間，檢測限量介於0.5~1.5 ppb間。本方法提供了快速、有效的檢驗方法，可減少人力及試驗耗費，增加檢驗的時效性。

關鍵字

液相層析串聯質譜法 ； 離子攜帶型抗生素 ； 雞蛋 ； 鮮乳

並列摘要

A LC/MS/MS method for the simultaneous detection and confirmation of five polyether ionophore antibiotics in egg and fresh milk was developed and validated. The analytes were extracted and injected into the LC-ESI-MS/MS system on a C18 column in gradient mode and an isocratic elution was performed. Results showed good repeatability, and recoveries for the five polyether ionophores used in egg and in milk averaged 90.5~95.0% and 95.0~104.7%, respectively. This method was suitable to confirm the presence of ionophore antibiotic residues at concentrations of 0.5~1.5 ppb in egg and fresh milk, respectively.

並列關鍵字

LC/MS/MS ； Ionophore antibiotics ； Eggs ； Milk