牛樟芝菌絲體及發酵萃取物對腸道α-葡萄糖苷酶抑制活性之研究

Inhibitory Effect on Intestinal α-Glucosidase by Extracts of Antrodia cinnamomea Mycelia and Cultural Filtrate Concentrate

黃贊勳(Tzann-Shun Hwang) ； 詹孟翰(Meng-Han Chan) ； 蔡文琦(Wen-Chi Tsai)

《臺灣農業化學與食品科學》 53卷4期 (2015/08) Pp. 171-176

https://doi.org/10.6578/TJACFS.2015.018

摘要

本研究探討以50%甲醇、80%甲醇及沸水萃取牛樟芝菌絲體及發酵液濃縮物，所得之萃取物對腸道α-葡萄糖苷酶之抑制活性。試驗結果顯示牛樟芝的菌絲體及發酵萃取物都能抑制腸道α-葡萄糖苷酶的活性，且隨著萃取物濃度越高，對腸道α-葡萄糖苷酶之抑制活性也越高。不論是菌絲體或發酵液皆以80%甲醇萃取物的抑制活性最高，菌絲體萃取物之半抑制濃度為310.3 mg/mL，發酵萃取物之半抑制濃度為284.0 mg/mL。以菌絲體80%甲醇萃取物進行酵素抑制動力學分析，結果顯示萃取物對腸道α-葡萄糖苷酶的抑制機制為非競爭型抑制作用。研究結果顯示牛樟芝應可經由抑制α-葡萄糖苷酶活性，有效應用於餐後血糖值的控制。

關鍵字

α-葡萄糖苷酶抑制劑 ； 牛樟芝菌絲體 ； 牛樟芝發酵液 ； 餐後高血糖

並列摘要

The aim of this study was to evaluate the inhibitory activities of methanol and hot water extracts of Antrodia cinnamomea mycelia and cultural filtrate concentrate on intestinal α-glucosidase activity. There was a dose dependent percentage inhibition by the extracts against intestinal α-glucosidase. The enzymatic assay showed that 80% methanol extracts of both mycelia and cultural filtrate were more potent against intestinal α-glucosidase activity than 50% methanol extract and aqueous extract. IC50 values of 310.3 mg/mL and 284.0 mg/mL for mycelia and cultural filtrate were obtained, respectively. The enzyme kinetic study revealed that 80% methanol extracts inhibited intestinal α-glucosidase activity noncompetitively. The results of the study therefore indicate the potential of these extracts to manage postprandial hyperglycemia via inhibiting intestinal α-glucosidase activity.

並列關鍵字

α-Glucosidase inhibitor ； Antrodia cinnamomea mycelia ； Antrodia cinnamomea cultural filtrate ； Postprandial hyperglycemia