A Water Extract of Pueraria Lobata Inhibited Cytotoxicity of Enterovirus 71 in a Human Foreskin Fibroblast Cell Line

葛根(Pueraria lobata)熱水萃取物在人類包皮纖維母細胞株抑制腸病毒71型造成的細胞毒性

蘇富敏(Fu-Min Su) ； 張榮參(Jung-San Chang) ； 王國治(Kuo-Chih Wang) ； 蔡季君(Jih-Jin Tsai) ； 蔣連財(Lien-Chai Chiang)

《The Kaohsiung Journal of Medical Sciences》 24卷10期 (2008/10) Pp. 523-530

摘要

Enterovirus 71 (EV71) can cause brain encephalitis and mortality. However, effective vaccines or chemotherapeutic agents are not yet available. We tested the hypothesis that Pueraria lobata could inhibit the cytotoxic effect of EV71 in a human foreskin fibroblast cell line by the XTT method. Our results showed that the water extract of P. lobata could inhibit cytopathy induced by EV71 when given before (p＜0.0001), simultaneously with (p＜0.0001), or after viral infection (p＜0.0001). Water extract of P. lobata was effective and its minimal concentration that inhibited 50% of the cytopathic effect (IC50) was 0.028 μg/mL. P. lobata was also safe with a selectivity index greater than 107,000. Water extract of P. lobata appeared to inhibit viral attachment (p＜0.0001) and penetration (p＜0.0001). The anti-EV71 activity of the water extract of P. lobata was not mediated by interferons. In conclusion, the water extract of P. lobata was effective in the management of the disease induced by EV71 infection.

關鍵字

化學預防 ； 腸病毒 ； 腸病毒71型 ； 葛根 ； 治療

並列摘要

Enterovirus 71 (EV71) can cause brain encephalitis and mortality. However, effective vaccines or chemotherapeutic agents are not yet available. We tested the hypothesis that Pueraria lobata could inhibit the cytotoxic effect of EV71 in a human foreskin fibroblast cell line by the XTT method. Our results showed that the water extract of P. lobata could inhibit cytopathy induced by EV71 when given before (p＜0.0001), simultaneously with (p＜0.0001), or after viral infection (p＜0.0001). Water extract of P. lobata was effective and its minimal concentration that inhibited 50% of the cytopathic effect (IC50) was 0.028 μg/mL. P. lobata was also safe with a selectivity index greater than 107,000. Water extract of P. lobata appeared to inhibit viral attachment (p＜0.0001) and penetration (p＜0.0001). The anti-EV71 activity of the water extract of P. lobata was not mediated by interferons. In conclusion, the water extract of P. lobata was effective in the management of the disease induced by EV71 infection.

並列關鍵字

chemoprevention ； enterovirus ； EV71 ； Pueraria lobata ； therapy