Using Enzyme Immunoassay to Detect Helicobacter Pylori Stool Antigen for Investigating the Prevalence of Helicobacter Pylori Infection in Children and Adolescents in Changhua

利用酵素免疫分析法偵測糞便中幽門螺旋桿菌抗原調查彰化地區兒童及青少年幽門螺旋桿菌感染之盛行率

鄭明宗(Ming-Tzong Cheng) ； 黃凱琳(Kai-Lin Hwang) ； 曹龍彥(Lon-Yen Tsao) ； 王昶皓(Chang-Hao Wang) ； 越文崇(Wun-Tsong Chaou) ； 張玉君(Yu-Jun Chang) ； 謝宜靜(Yi-Ching Hsieh)

《Acta Paediatrica Taiwanica》 43卷3期 (2002/06) Pp. 133-139

https://doi.org/10.7097/APT.200206.0133

摘要

為了調查彰化地區兒童及青少年幽門螺旋桿菌感染之盛行率，並比較城鄉之差異，我們利用酵素免疫分析法偵測糞便中之幽門螺旋桿菌抗原，以作為糞便盛行率之調查，並設計問卷以取得人口統計學及監床資訊。在彰化市及永靖鄉18歲以下兒童及青少年每3歲為一組，分成六組即0-3,3-6,6-9,9-12,12-15，及15-18歲，每組最少30人，總共567人（男生／女生295/272）。結果發現，幽門螺旋桿菌感染與居住地（城鄉）、性別、父母新職業、母親教育程度、家人潰瘍史、及家中成員人數無相關性，但是與上腹痛成正相關（P值＝0.001）與父親教育程度成逆相關（P值＝0.039）。整體盛行率為13.75%,0-3歲組最低(2%)。盛行率隨年紀增加而增高，直到9-12歲達23.8％。經複迴歸分析後發現年紀及上腹痛為最重要之危險因子。本研究在幽門螺旋桿菌感染年紀之特性與其他研究之差異仍有待進一步探討。

關鍵字

酵素免疫分析法 ； 糞便幽門螺旋桿菌抗原 ； 上腹痛 ； 糞便盛行率

並列摘要

To investigate the prevalence of Helicobacter pylon (H. pylon) infection in adolescents and children in Changhua and to compare the differences between urban and rural areas, we used enzyme immunoassay to detect H. pylon stool antigen for feco-prevalence study. In addition, a questionnaire was designed to obtain demographic and clinical information. Children and adolescents under 18 years of age living in Changhua city and Yung-Chin village were divided into 6 groups respectively, i.e. 0-3, 3-6, 6-9, 9-12, 12-15, and 15-18 years. A minimum of 30 persons were investigated in each group. A total of 567 subjects (M/F 295/2 72) were included in this study. There was no significant difference in H. pylon infection with regard to residential area, gender education of the mother, occupation of parents, family history of peptic ulcer, and number of family members, except for epigastralgia of the subjects (p=0.001) and education of the father (p=0.039). The overall prevalence was 13.75%. A low prevalence was noted in the group 0-3 years (2.0%). The prevalence increased with age and reached it’s maximum of 23.8% in the age group of 9-12 years and then declined. The multiple logistic regression analysis revealed that only age [odds ratio(OR)=1.3, 95% confidence interval(C.I.)=1.1-1.5] and epigastralgia (OR=1.71, 95% C.I.=1.0-3.0) were the most important risk factors for H. pylon infection after adjusting for participating gender, place of residence and father’s age. The difference in age pattern of H. pylon infection between our results and other studies needs further investigation. (Acta Paediatr Tw 2002; 43:133-9)

並列關鍵字

enzyme immunoassay ； Helicobacter pylon stool antigen ； prevalence ； epigastralgia